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Testing a non-lethal DNA isolation technique for freshwater mussels that is compatible with polymerase chain reaction (PCR) based studies.(deoxyribonucleic acid)

Transactions of the Kansas Academy of Science

| March 22, 2007 | Spicer, Judson F., Jr.; Sobieski, Rodney J.; Crupper, Scott S. | COPYRIGHT 2007 Kansas Academy of Science. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan.  All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)Copyright

In this study, a non-lethal DNA isolation technique was developed for freshwater mussels. A total of 45 Quadrula quadrula, 21 Q. metanevra, and 19 Q. pustulosa were collected from the Neosho and Verdigris Rivers in eastern Kansas. DNA was successfully isolated from 82 mussels using the non-lethal technique developed in this study. Spectrophotometer analysis of the 82 DNA samples resulted in an average DNA concentration of 488 ng/[micro]l. Agarose gel electrophoresis of polymerase chain reaction products obtained using the purified mussel DNA demonstrated high quality, reproducible amplification products. All mussels remained alive for several months after tissue biopsy in ...

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Source: HighBeam Research, Testing a non-lethal DNA isolation technique for freshwater mussels...

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