Production of IL-1 beta and IL-6 but not TNF-alpha is enhanced by LPS stimulus in Mycobacterium avium infected human macrophages. (interleukin, tumor necrosis factor) (Research Report)

According to an abstract submitted by the authors to the Conference on Frontiers in Mycobacteriology and Multi-Drug-Resistant Tuberculosis, held October 12-16, 1994, in Vail, Colorado, "In order to investigate whether the infection with Mycobacterium avium can modify the response of human macrophages (MP) to LPS, infected MP and uninfected controls were treated at different times (from 0 up to 8 days) with 10 mg/ml LPS, and the release of cytokines, namely IL-1b, IL-6 and TNF-a was determined by ELISA after 18 h of LPS addition. Transparent colonies of the strain 485 (type 21) were used to infect MP; during the time of observation the number of the intracellular bacteria …