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Tests for antibodies against bovine herpesvirus 1 evaluated.
2004 DEC 1 - (NewsRx.com & NewsRx.net) -- Researchers have evaluated tests for antibodies against bovine herpesvirus 1 performed in national reference laboratories in Europe.
"Sets of serum and milk samples were collected from various countries and prepared, lyophilized, and distributed by one laboratory to 12 reference laboratories in Europe. The serum sets contained the three European bovine herpesvirus 1 (BHV1) reference serum samples (EU1, EU2, and EU3), serum samples from naturally and experimentally BHV1-infected cattle, from vaccinated, and vaccinated-challenged cattle, from uninfected cattle, and a series of serum dilutions. In addition, sets of milk samples were distributed," scientists in the Netherlands report.
"The samples were tested for antibodies against BHV1 in virus neutralization tests, in gB-specific ELISAs, in indirect ELISAs, and in gE-specific ELISAs," said Johannes A. Kramps at the University of Wageningen in the Netherlands and collaborators throughout Europe. "It was found that the virus neutralization test and the gB-specific ELISAs were most sensitive for the detection of antibodies in serum, whereas for assaying milk samples the indirect ELISAs were the tests of choice. The results show that the quality of most ...
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