Repeated boosting with E. coli bacterin needed for high antibody levels.

2004 DEC 1 - (NewsRx.com & NewsRx.net) -- Repeated boosting with J5 E. coli bacterin is required to generate high levels of cross-reactive IgG[subscript]2 antibodies.

According to published research from the United States, "Isotype-specific antibody responses and cross reactivity were profiled following hyperimmunization of steers with J5 Escherichia coli bacterin. The vaccine was administered at time 0, 30 days later, and every two weeks for 10 subsequent immunizations. Blood was collected preimmunization and multiple times following each immunization. Isotype-specific anti-J5 E. coli antibody response profiles in diluted sera harvested from each sample were assayed by ELISA and recorded as optical density."

"Selected sera were assayed for anti-J5 E. coli antibody titers and used to determine cross reactivity against a variety of Gram-negative bacteria," said A. Chaiyotwittayakun and colleagues at Michigan State University. "Immunization number and day postimmunization influenced response profiles for anti-J5 E. coli IgM, IgG[subscript]1, and IgG[subscript]2 antibodies. Two immunizations increased mean serum IgM and the IgG[subscript]1 antibody profiles above preimmunization levels, but five immunizations were required to detect significant IgG[subscript]2 antibody responses that were above preimmunization levels."

"Isotype-specific cross reactivity of the serum antibodies with a ...