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Whole blood cytokine detection assay to quantitate T-cells developed.

Vaccine Weekly

| December 01, 2004 | COPYRIGHT 2004 NewsRX. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan.  All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)Copyright

2004 DEC 1 - (NewsRx.com & NewsRx.net) -- Scientists have developed a whole blood intracellular cytokine detection assay to quantitate specific T-cell frequency in field studies.

According to recent research from the United States and South Africa, "We optimized a whole blood intracellular cytokine assay to quantitate the frequency of specific CD4+ and CD8+ T cells in small volumes of whole blood from infants from developing countries. The assay is performed in two steps. First, whole blood is stimulated in the presence of specific antigens for 6-18 hours, ending with cryopreservation of fixed white cells."

"These stimulation steps were specifically adapted to be practical and reliable in a rural, developing country field setting," said Willem A. Hanekom at the University of Miami in the U.S. and collaborators in the U.S. and South Africa. "Later, in a more resourceful setting, interferon-gamma producing CD4+ or CD8+ T cells are detected by flow cytometry. The assay proved sensitive and specific for detecting mycobacteria-specific ...

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