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Retroviral vectors used to analyze SIV/HIV-specific T cell responses.
2004 DEC 1 - (NewsRx.com & NewsRx.net) -- Researchers have used retroviral vectors for the analysis of SIV/HIV-specific CD8 T cell responses.
"CD8+ T cell responses and particularly cytotoxic T lymphocyte (CTL) activity are critical factors in controlling SHIV, SIV or HIV replication during natural infection and represent key parameters which need to be monitored during vaccine development. In order to improve the methodology for measuring CD8+ T cell responses, retroviral vectors expressing the fall-length SIV-Gag or HIV-Env proteins were constructed and used to transduce B lymphoblastoid cell lines (BLCL) from cynomolgus monkeys infected with SHIV89.6P," scientists writing in the Journal of Immunological Methods report.
"Continuous expression of Gag and Env proteins was detected in stably transduced BLCL, which induced Gag- or Env-specific T cell responses, as measured by both IFN-gamma/ELISPOT and chromium release assays, upon in vitro stimulation of PBMC from the SHIV89.6P-infected monkeys," stated Donatella R. M. Negri and colleagues at the Istituto Superiore di Sanita and the University of Padua in Italy. "Moreover, induction of Gag-specific CTL using BLCL transduced with retroviral vector expressing the SIV-Gag protein was more efficient and specific compared to that obtained using BLCL infected with a recombinant vaccinia virus (rVV) encoding for the same antigen."
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Source: HighBeam Research, Retroviral vectors used to analyze SIV/HIV-specific T cell responses.