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Polycation-mediated precipitation captures plasmid DNA from alkaline lysate.
2004 NOV 3 - (NewsRx.com & NewsRx.net) -- Polycation-mediated precipitation captures plasmid DNA from alkaline lysate.
"The demand for highly purified plasmids in gene therapy and plasmid-based vaccines requires large-scale production of pharmaceutical-grade plasmid. Large-scale purification of plasmid DNA from bacterial cell culture normally includes one or several chromatographic steps. Prechromatographic steps include precipitation with solvents, salts, and polymers combined with enzymatic degradation of nucleic acids," scientists in Sweden report.
"No method alone has so far been able to selectively capture plasmid DNA directly from a clarified alkaline lysate," according to P.O. Wahlund and colleagues, Lund University, Center for Chemistry and Chemical Engineering.
"We present a method for selective precipitation of plasmid DNA from a clarified alkaline lysate using polycation poly(N,N'-dimethyldiallylammonium) chloride (PDMDAAC). The specific interaction between the polycation and the plasmid DNA resulted in the formation of a stoichiometric insoluble complex.
"Efficient removal of contaminants such as ...
- Erythrocyte ghosts prolong plasmid DNA expression in the blood.
- Newspaper article from: Biotech Week April 7, 2004 700+ words
...net) -- Erythrocyte ghosts prolong plasmid DNA expression in the blood. According...circulation and prolonged expression of plasmid DNA in the blood. Murine interleukin-2-expressing plasmid DNA was efficiently loaded to EG by electroporation...
- Inoculation of plasmid DNA induced expression of LacZ gene.
- Newspaper article from: Biotech Week June 23, 2004 700+ words
...net) -- Intramuscular inoculation of plasmid DNA induced the expression of the LacZ gene in...responses to the antigen encoded by cDNA in a plasmid DNA. In the present study, a plasmid DNA encoding cDNA of beta-galactosidase (beta...
- Plasmid DNA the High-Throughput Way.
- Magazine article from: The Scientist WOLF, PAUL G. January 22, 2001 700+ words
...appropriate quality, quantity, and purity of plasmid DNA, the starting material for many lab...feasible to manually extract high-quality plasmid DNA from several hundred samples in a day...unattended. If large quantities of plasmid DNA are needed, manual HT preps become...
- Plasmid DNA activates dendritic cells via toll-like receptor 9.
- Newspaper article from: Biotech Week January 21, 2004 700+ words
...NewsRx.net) -- Vaccination with plasmid DNA activates dendritic cells via toll...whether the immunobiology of vaccinating plasmid DNA containing a transcription unit for...motifs in the plasmid backbone. Indeed, plasmid DNA differentially activated in vitro myeloid...
- Microparticles act as carriers to deliver plasmid DNA in vaccines.
- Newspaper article from: Biotech Week August 4, 2004 700+ words
...as carriers for the delivery of plasmid DNA for vaccine development. According...study from the United States, "Plasmid DNA was directly encapsulated into...demonstrated. By trapping the plasmid DNA within the cross-linked microparticle...
- Automated plasmid DNA purification.(Automation/Robotics Focus)
- Magazine article from: Bioscience Technology October 1, 2004 700+ words
...enables reproducible purification of plasmid DNA that is ready for direct use in high...enables purification of sequencing grade plasmid DNA from 96 samples per run. Typically, yields of up to 4 [micro]g high-copy plasmid DNA can be obtained from 1.25 ml E. coil...
- H. Lee Moffitt Cancer Center and Inovio Biomedical Announce Positive Results...
- Press release article from: Business Wire June 1, 2006 700+ words
...DNA Electroporation System to deliver plasmid DNA to tumors with the aim of treating malignant...s electroporation system to deliver plasmid DNA encoding a cytokine, interleukin...method permits efficient expression of plasmid DNA, with demonstrable immune responses...
- M. leprae Hsp65-encoding plasmid DNA induces anti-mycobacterial immunity.
- Newspaper article from: Tuberculosis Week March 15, 2004 700+ words
...Mycobacterium leprae Hsp65-encoding plasmid DNA in biodegradable microparticles induces...single sub-cutaneous injection of a plasmid DNA encoding a mycobacterial heat shock...vaccination coverage with candidate plasmid DNA vaccines, especially in developing...
- Plasmid DNA integrates into host genomic DNA after injection.
- Newspaper article from: Gene Therapy Weekly June 10, 2004 700+ words
...NewsRx.net) -- Integration of plasmid DNA into host genomic DNA was detected following...definitive demonstration of integration of plasmid DNA into genomic DNA following injection...Therapy (Detection of integration of plasmid DNA into host genomic DNA following intramuscular...
- Plasmid DNA targets the kidneys after retrograde injection into the renal vein.
- Newspaper article from: Biotech Week March 24, 2004 700+ words
...NewsRx.com & NewsRx.net) -- Plasmid DNA targets the kidneys after retrograde...retrograde renal vein injection of naked plasmid DNA. Many animal disease models are created...demonstrate that the kidney-targeted naked plasmid DNA transfer of mice by retrograde renal...
Source: HighBeam Research, Polycation-mediated precipitation captures plasmid DNA from alkaline...