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Cholesteryl ester transfer protein induces long-lasting antibodies.
2004 NOV 3 - (NewsRx.com & NewsRx.net) -- Subcutaneous and mucosal administration of a 26-amino acid cholesteryl ester transfer protein (CETP) epitope carried by heat shock protein 65 kDa in the absence of adjuvants induced long-lasting specific antibodies against CETP.
"The heat shock protein 65 kDa (Hsp65) of Mycobacterium tuberculosis var. bovis was fused with the linear polypeptide epitope of cholesteryl ester transfer protein C-terminal fragment (CETPC) and expressed as soluble protein in Escherichia coli. The fusion protein Hsp65-CETPC was purified by anion exchange column and eluted at 100-130 mM NaCl in 10 mM phosphate buffer (pH 8.0), and then used to immunize mice via subcutaneous injection or intranasal delivery in the absence of adjuvants," scientists in China report.
"Antibodies against CETPC were detected in immunized mice sera by enzyme-linked immunosorbent assay (ELISA) and verified by Western blot analysis," said Qi Gaofu and collaborators at China Pharmaceutical University. "Specific antibodies were successfully induced and lasted for more than 12 weeks in animals immunized with the fusion protein via both subcutaneous and intranasal routes even in the absence of adjuvants."
The researchers reported, "Results showed that Hsp65 could be used as a convenient carrier ...
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