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Microneutralization assay using cytomegalovirus strains developed.
2004 NOV 3 - (NewsRx.com & NewsRx.net) -- Scientists have developed an efficient fluorescence-based microneutralization assay using recombinant human cytomegalovirus strains expressing green fluorescent protein.
"MedImmune Vaccines has created four, live, attenuated human cytomegalovirus (HCMV) vaccine candidates, each derived from defined portions of the parental strains, Towne and Toledo. To determine each candidate's ability to induce HCMV specific immunity, a fluorescence-based microneutralization assay was developed using recombinants of Toledo and Towne, which express enhanced green fluorescent protein (EGFP). Replication of the EGFP recombinants in cell culture was the same as the respective parental strains," researchers in the United States report.
"Using the EGFP recombinants, this fluorescence-based microneutralization assay was compared with the traditional plaque reduction assay," said Zhaoti Wang and colleagues at MedImmune Vaccines Inc. "Serum samples were analyzed by both the fluorescence microneutralization and plaque reduction assays and regression analysis showed a correlation of r[superscript]2greater than or equal to0.90 between the two assays. As an alternative to measuring fluorescence, infected cells were examined microscopically and the number of green fluorescent cells was counted automatically. Regression lines between fluorescent cell counting and fluorescence in the well also showed a high correlation (r[superscript]2greater than or equal to0.92)."
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Source: HighBeam Research, Microneutralization assay using cytomegalovirus strains developed.