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Extraction method to measure halogenated compounds in breast milk developed.
2004 OCT 7 - (NewsRx.com & NewsRx.net) -- Scientists have developed a semiautomated high-throughput extraction and cleanup method for the measurement of polybrominated diphenyl ethers and polybrominated and polychlorinated biphenyls in breast milk.
"A semiautomated extraction and cleanup method has been developed to measure eight polybrominated diphenyl ethers (PBDEs), 2,2',4,4',5,5'-hexabromobiphenyl (BB-153), and 2,2',4,4',5,5'-hexachlorobiphenyl (CB-153). The method employs solid-phase dispersion on diatomaceous earth in a solid-phase extraction cartridge followed by automated addition of internal standards ([superscript]13C-labeled). Extraction is then performed using an automated modular solid-phase extraction system," investigators in the United States report.
"The extraction procedure includes drying the sample on diatomaceous earth by pressurized nitrogen and eluting target analytes and lipids with dichloromethane," said Andreas Sjodin and colleagues at the U.S. Centers for Disease Control and Prevention. "Lipid content is determined gravimetrically. Lipid determinations performed using this method are compared with other standard methods and with a certified reference material. A relative standard deviation of 7.9% was obtained for 130 determinations of the lipid content in a breast milk quality control sample. Final analytical determination of target analytes was performed by gas chromatography-isotope dilution high-resolution ...
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Source: HighBeam Research, Extraction method to measure halogenated compounds in breast milk...