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Vaginal, cervical cancer differentiated by cluster analysis of protein patterns.
2004 OCT 7 - (NewsRx.com & NewsRx.net) -- Cluster analysis of proteomics data differentiates among normal vaginal mucosa, primary vaginal and primary cervical cancer
"Protein patterns in six samples from primary vaginal cancers, in five from normal vaginal tissue, and in five primary cervical cancers, were analyzed using two-dimensional polyacrylamide gel electrophoresis (2-DE)," explained K. Hellman and colleagues at Karolinska Hospital.
"Protein expression profile was evaluated by computer-assisted image analysis (PDQUEST) and proteins were subsequently identified using matrix-assisted laser desorption/ionization mass spectrometry. The aim was to analyze the protein expression profiles using the hierarchical clustering method in vaginal carcinoma and to compare them with the protein pattern in cervical carcinoma in order to find a helpful tool for correct classification and for increased biomedical knowledge," the scientists wrote.
"Protein expression data of a distinct set of 33 protein spots were differentially expressed. These differences were statistically significant (Mann-Whitney signed-Ranked Test, p
"Furthermore," they said, "protein profiles of pairs of primary vaginal and cervical cancers were found to be very similar. Some of the protein spots that have so far been identified include Tropomyosin 1; cytokeratin 5, 15, and 17; Apolipoprotein A1; Annexin V; Glutathione-S-transferase. Others are the stress-related proteins calreticulin, HSP 27, and ...
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Source: HighBeam Research, Vaginal, cervical cancer differentiated by cluster analysis of...