AccessMyLibrary provides FREE access to over 30 million articles from top publications available through your library.
Create a link to this page
Copy and paste this link tag into your Web page or blog:
Ubiquitin-fusion degradation pathway plays key role in DNA vaccination.
2004 OCT 6 - (NewsRx.com & NewsRx.net) -- The ubiquitin-fusion degradation pathway plays an indispensable role in naked DNA vaccination with a chimeric gene encoding a syngeneic cytotoxic T lymphocyte epitope of melanocyte and green fluorescent protein.
According to published research from Japan, "Antitumor immunity against murine melanoma B16 was achieved by genetic immunization with a naked chimeric DNA encoding a fusion protein linking green fluorescent protein (GFP) to the N-terminus of a major CD8+ cytotoxic T lymphocyte (CTL) epitope of tyrosinase-related protein 2 (TRP-2[subscript]181-188) of murine melanoma, designated as pGFP-TRP-2. Tumor growth was profoundly suppressed in C57BL/6 mice immunized with pGFP-TRP-2, while mice vaccinated with pTRP-2 showed rapid tumor growth and died within 40 days after tumor challenge. Splenocytes of mice immunized with pGFP-TRP-2 showed high CTL activity specific for TRP-2[subscript]181-188."
"GFP-TRP-2 expressed in COS-7 cells was rapidly degradated in vitro and the degradation was almost completely prevented by adding a proteasome inhibitor, MG-132, in the culture," said Manxin Zhang and collaborators at Kyushu University and the Tokyo Metropolitan Institute of Medical Science. "Furthermore, the antimelanoma immunity induced by genetic immunization with pGFP-TRP-2 was completely cancelled in mice deficient in proteasome activator PA28alpha/beta. Taken together, GFP-TRP-2 processed by cytosolic proteasome played a central role in breaking peripheral tolerance to a melanoma/melanocyte antigen, TRP-2[subscript]181-188, by activating CD8+ CTL specific for TRP-2[subscript]181-188."
"TRP-2[subscript]181-188 fused to GFP may be ...
- Is the TRP the defense conversion cure-all? (Technology Reinvestment Project)
- Magazine article from: Mechanical Engineering-CIME Becker, Pamela October 1, 1994 700+ words
...announced the Technology Reinvestment Project (TRP) as the centerpiece of the administration...initial launch amid presidential fanfare, the TRP has had a mixed reception. Some believe...Research Projects Agency (ARPA), the TRP is currently being funded at probationary...
- Time running out for TRP; Kaminski advised to pick and choose.(Technology...
- Newspaper article from: Defense Daily Gildea, Kerry May 18, 1995 700+ words
...decide what Technology Reinvestment Program (TRP) projects must survive and which can perish...a "heads up" to take a careful look at TRP before the budget axe falls. "You should...urging the panel members to fully fund TRP at $500 million, said a severe funding...
- Dendreon Presents Preclinical Data Demonstrating Activity of Trp-p8 agonist,...
- Press release article from: PR Newswire May 21, 2008 700+ words
...bioavailable small molecule, which targets Trp-p8 (a transmembrane cation channel protein also known as Trp-M8), to treat benign prostatic hyperplasia...2041), "Preclinical Validation of the Trp-p8 Ion Channel as a Target for Benign Prostatic...
- Services directed to escalate TRP involvement to reap benefits. (Technology...
- Newspaper article from: Defense Daily August 9, 1994 700+ words
...administration's Technology Reinvestment Program (TRP) in order to protect DoD's technology...Pentagon officials have complained that TRP is expanding at the expense of too many...Department must receive some benefit from the [TRP] investment of $625 million," the appropriators...
- Dendreon Corporation and Genentech to Collaborate on Trp-p8 Gene Platform --...
- Press release article from: Business Wire August 13, 2002 700+ words
...commercialization of products derived from Dendreon's Trp-p8 gene platform. The agreement concerns...other products derived from Dendreon's Trp-p8 gene platform. In addition to the...million for the resulting development of Trp-p8 products. The companies will be jointly...
- Dendreon Presents Preclinical Data Validating Anti-Tumor Activity of D-3263, a...
- Press release article from: PR Newswire October 23, 2008 700+ words
...preclinical data on its lead small molecule candidate, D-3263, which targets Trp-p8 (a transmembrane cation channel protein also known as Trp-M8), supporting Trp-p8 as a therapeutic cancer target. The poster, titled, "Preclinical Validation...
- TRP heavily military-oriented, report says.(National Commission for Economic...
- Newspaper article from: Defense Daily July 14, 1995 700+ words
...administration's Technology Reinvestment Plan (TRP), designed to promote dual-use technology...Hill as a non-military use of DoD money, TRP has actually failed in its mission to foster...Disarmament (ECD) of Washington, D.C. TRP, the report says, has only "modest potential...
- Dendreon's Trp-p8 Gene Platform Garners $110M Genentech Deal.
- Magazine article from: BIOWORLD Today November 4, 2009 700+ words
...other products from Dendreon's preclinical Trp-p8 gene platform. The Street viewed the...provides Genentech with exclusive access to Trp-p8," Page Sargisson, spokeswoman for...110 million for resulting development of Trp-p8 products. The companies jointly will...
