AccessMyLibrary provides FREE access to over 30 million articles from top publications available through your library.
Create a link to this page
Copy and paste this link tag into your Web page or blog:
Anti-atherosclerosis polypeptide vaccine expressed in E. coli.
2004 OCT 6 - (NewsRx.com & NewsRx.net) -- Scientists have expressed an anti-atherosclerosis polypeptide vaccine in Escherichia coli.
According to recent research from China, "A chimeric polypeptide of TTP-CETPC was successfully expressed as inclusion bodies in Escherichia coli by fusing it with the C-terminus of asparaginase and a basic amino acid-rich peptide (KR). After partially purified by washing with 0.5% (v/v) Triton X-100 in 10 mM PB, the pellet was solubilized in 8 M urea. The solution was precipitated with single volume and double volumes of cold ethanol for removing impurities."
"The fusion protein in solution was precipitated with triple volumes of ethanol to increase purity and then hydrolyzed with 50 mM hydrochloric acid at 55 degrees C for 72 hours," reported Gaofu Qi and colleagues at China Pharmaceutical University. "The TTP-CETPC polypeptide was released after the unique acid-labile aspartylprolyl bond in the fusion protein was cleaved by acid. After impurities were removed by adjusting the hydrolysis solution pH to 9.45 and then to 8.37, the TTP-CETPC polypeptide was further purified by DEAE-cellulose column. The TTP-CETPC containing fractions were eluted at 60-80 mM NaCl."
"The purified TTP-CETPC cysteines were ...
- Invitrogen Announces the Launch of Expressway(TM) In Vitro Protein Expression...
- Press release article from: PR Newswire January 9, 2002 700+ words
...of its Expressway(TM) In Vitro Protein Expression System, a rapid method for producing...scientific procedures." In vitro protein expression systems that allow expression of...The Expressway(TM) In Vitro Protein Expression System yields up to 50 micrograms...
- Lung Cancer Study at M.D. Anderson Combines Gene Expression and Protein...
- Press release article from: PR Newswire April 7, 2003 700+ words
...describing a study in which gene and protein expression profiling were combined to reveal...Center), used complementary gene and protein expression profiling tools. Affymetrix's...monitor cellular changes in gene and protein expression and discover the molecular targets...
- RiNA GmbH Acquires Roche RTS Protein Expression Portfolio.
- Newspaper article from: Biotech Week September 23, 2009 700+ words
...complete Roche Applied Science RTS Protein Expression portfolio to RiNA GmbH, effective...attractive market for cell-free protein expression with a well established product line...field of prokaryotic and eukaryotic protein expression systems. RiNA has been working closely...
- Companies Rely on RCT Protein Expression Technology to Make Therapeutic...
- Press release article from: Business Wire November 27, 2006 700+ words
...sequence that enables high levels of protein expression in mammalian cells is in use worldwide...lymphoma and rheumatoid arthritis. For protein expression to occur in mammalian cells, messenger...translation and stabilizes the mRNA during protein expression. Fritz Rottman, Ph.D., ...
- Ciphergen announces protein expression profiling patent issuance.(Brief Article)
- Newspaper article from: BIOTECH Patent News May 1, 2001 700+ words
...225,047, directed to a novel protein expression profiling method for protein biomarker...System are already using this powerful protein expression profiling method to discover and...Retentate Chromatography method of protein expression profiling, proteins from different...
- Bcl-2 and Bax protein expression in lymphocytic cells aren't interindependent.
- Newspaper article from: Cancer Weekly December 10, 2002 700+ words
...sequences were able to reduce Bcl-2 protein expression in B-cell chronic lymphocytic leukemia...determine whether Bcl-2 and Bax protein expression were interdependent upon one another...oligonucleotide down-regulated Bax protein expression between 23.7 and 68.8% in the...
- Roche Protein Expression Group Helps Accelerate the Path to Drug Discovery;...
- Press release article from: PR Newswire August 11, 2003 700+ words
...announced today the creation of the Roche Protein Expression Group (RPEG), a business unit...difficult to express by conventional protein expression technology. We aim to deliver the...Development for the RPEG. The Roche Protein Expression group leverages more than 20 years...
- Differential analysis identifies all disease-related protein expression changes.
- Newspaper article from: Clinical Oncology Week March 17, 2003 700+ words
...identifies all disease-related protein expression changes. According to a study from...step, we analyzed the differential protein expression of liver tumor samples, to find...analyzed for their 2-DE profiles. Protein expression was comparatively analyzed by use...
Source: HighBeam Research, Anti-atherosclerosis polypeptide vaccine expressed in E. coli.