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2004 SEP 18 - (NewsRx.com & NewsRx.net) -- Culture systems improve the study of gene function in inaccessible tissue.
"The development of an organ culture system for growing prenatal intercostal muscle in vitro and its use to study gene function is described. Fetal skeletal muscle is relatively inaccessible during the key stages of its development, and this method enables DNA transfections and other manipulations to be carried out. The system allows cell proliferation and differentiation to continue and also maintains the morphology and fiber types of developing muscle," scientists writing in the journal Muscle & Nerve report.
"Gene transfer into cultured embryonic intercostal muscle was achieved by square-pulse electroporation of intact pieces of tissue. Expression of a marker gene green fluorescent protein (GFP) was found within 5 hours and maintained for 2 days in muscle fibers and cells," wrote S.M.P. Cardoso and colleagues, University of Nottingham Hospital, Queens Medical ...