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Two antigenic epitopes on the SARS-CoV spike protein are identified.
2004 SEP 1 - (NewsRx.com & NewsRx.net) -- Two antigenic epitopes on severe acute respiratory syndrome-coronavirus (SARS-CoV) have been identified.
According to a study from the People's Republic of China, "The spike (S) protein of severe acute respiratory syndrome-coronavirus (SARS-CoV) is a major virion structural protein. It plays an important role in interaction with receptor and inducing neutralizing antibodies.
"In the study, six tentative antigenic epitopes (S1 S2 S3 S4 S5 S6) of the spike protein of SARS-CoV were predicted by bio-informatics analysis, and a multi-epitope chimeric gene of S1-S2-S3-S4-S5-S6 was synthesized and fused to downstream GST gene in pGEX-6p-1."
R. Hua and coworkers explained, "The Western blotting demonstrated that SARS patient convalescent serum could recognize the recombinant fusion protein. A number of monoclonal antibodies were developed against the fusion protein.
"[F]urther, the six predicted epitope genes were individually fused to GST of pGEX-6p-1 and expressed in Escherichia coli BL21, respectively. Among six fusion peptides, S5 reacted with monoclonal antibody D3C5 and S2 reacted with monoclonal antibody D3D1 against spike protein of SARS-CoV."
The researchers concluded, "The epitopes recognized ...
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Source: HighBeam Research, Two antigenic epitopes on the SARS-CoV spike protein are identified.