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Method to quantify mumps virus RNA by one-step real-time RT-PCR developed.
2004 SEP 1 - (NewsRx.com & NewsRx.net) -- Researchers have developed a method for the rapid and quantitative detection of mumps virus RNA by one-step real-time RT-PCR.
"We developed a new TaqMan-based one-step real-time reverse transcription-polymerase chain reaction (RT-PCR) assay for detection and quantification of mumps virus RNA. Oligos targeting the matrix protein gene of mumps virus were designed by using our oligo designing and analyzing software, Oligoware 1.0. Oligos's specificity was tested with five strains (four laboratory isolated and one Jeryl Lynn strain) of mumps virus. The suggested TaqMan-based one-step real-time RT-PCR assay correctly detected the four laboratory-isolated strains and one Jeryl Lynn strain," investigators in Turkey report.
"To confirm the specificity of the TaqMan PCR assay, parainfluenza type 1, 2, 3 strains, sendai virus, and measles virus (vaccine strain) were tested, and no cross-reactivity was observed between mumps and tested strains," reported Ayhan Kubar and colleagues at Gulhane Military Medical Academy. "In addition, a BLAST (NCBI) search showed no genomic cross-reactivity with other viruses or cells. Testing of the assay's reproducibility was repeated several times, and the same results were achieved. The new assay was able to quantify the concentrations of mumps virus gene ranging from 10[superscript]1 to 10[superscript]8 copies per reaction sensitively with generated plasmid standards."
"In addition, it was shown that a ...
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Source: HighBeam Research, Method to quantify mumps virus RNA by one-step real-time RT-PCR...