Tetramers with influenza hemagglutinin and EBV epitopes detect CD4+ T cells.

2004 SEP 1 - (NewsRx.com & NewsRx.net) -- MHC class II tetramers containing influenza hemagglutinin and Epstein-Barr virus (EBV) EBNA1 epitopes detect reliably specific CD4+ T cells in healthy volunteers.

According to recent research from the United States, "Tracking antigen-specific T cells with major histocompatibility complex (MHC) tetramers has provided us with insights into the dynamics of the adaptive immune system and holds great promise to aid in patient management and drug and vaccine development. Progress has been made primarily using MHC class I tetramers to monitor CD8+ T cells, whereas corresponding efforts to stain CD4+ T cells with class II tetramers have not been as successful.

"Two major reasons have been proposed for this lack of progress: the frequency of antigen-specific CD4+ T cells is lower than the frequency of CD8+ T cells; and some, but not all, antigen-specific CD4+ T cells can bind tetramer because of low functional avidity."

"In this study, we asked if CD4+ T cells specific for common human viruses (e.g., influenza and Epstein-Barr) can be detected in healthy individuals previously exposed to them," said Ming Ye and colleagues at Beckman Coulter, Inc. "We were able to clearly detect specific CD4+ T cells in all donors after in vitro expansion ...