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Prostaglandin E[subscript]2 receptors up-regulated in peritoneal macrophages.
2004 SEP 1 - (NewsRx.com & NewsRx.net) -- Prostaglandin E[subscript]2 receptors EP2 and EP4 are up-regulated in peritoneal macrophages and joints of pristane-treated mice and modulate TNF-alpha and IL-6 production.
"Prostaglandin E[subscript]2 (PGE[subscript]2) can have pro-or anti-inflammatory effects, depending on engagement of different PGE[subscript]2 receptor (EP) subtypes. The role of EPs in regulating autoimmune inflammation was studied in the murine arthritis/lupus model induced by pristane. Peritoneal macrophages were isolated (biomagnetic beads) from BALB/c, DBA/1, or C57BL/6 mice treated with pristane (intraperitoneally, 3 months earlier) or thioglycolate (3 days earlier) or with untreated controls. EPs, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) mRNA expression was examined by reverse transcriptase-polymerase chain reaction (RT-PCR)," scientists writing in the Journal of Leukocyte Biology report.
"Cells were cultured unstimulated or stimulated with lipopolysaccharide (LPS) or LPS + interferon-gamma in combination with EP subtype-specific agonists," said Jun Akaogi and colleagues at the University of Florida. "Tumor necrosis factor alpha (TNF-alpha) and interleukin (IL)-6 production was tested by enzyme-linked immunosorbent assay (culture supernatant) and flow cytometry. TNF-alpha mRNA levels also were examined. High levels of EPs (EP4/2>EP1>EP3), iNOS, and COX-2 mRNA were expressed in peritoneal macrophages from pristane-treated but not untreated or thioglycolate-treated mice (RT-PCR). TNF-alpha production was inhibited 50-70% at 2-24 hours by EP4/2 agonists, whereas IL-6 was enhanced up to (approx)220%."
"TNF-alpha inhibition is mediated ...
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Source: HighBeam Research, Prostaglandin E[subscript]2 receptors up-regulated in peritoneal...