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Responses to antigen of Plasmodium linked to malaria immunity.

Vaccine Weekly

| August 11, 2004 | COPYRIGHT 2004 NewsRX. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan.  All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)Copyright

2004 AUG 11 - (NewsRx.com & NewsRx.net) -- Responses to the rhoptry-associated membrane antigen of Plasmodium falciparum are associated with immunity to malaria infection.

"Rhoptry proteins participate in the invasion of red blood cells by merozoites during the malaria parasite's asexual-stage cycle. Interference with the rhoptry protein function has been shown to prevent invasion, and three rhoptry proteins have been suggested as potential components of a vaccine against malaria. Rhoptry-associated membrane antigen (RAMA) is a 170-kDa protein of Plasmodium falciparum which is processed to a 60-kDa mature form in the rhoptries," scientists in Australia, the U.S., and Vietnam report.

"p60/RAMA is discharged from rhoptries of free merozoites and binds to the red-cell membrane before being internalized to form part of the parasitophorous vacuole of the newly developing ring," said Agnieszka E. Topolska at Monash University and collaborators in Australia, the U.S., and Vietnam. "We examined the range of anti-RAMA responses in individuals living in an area of endemicity for malaria and determined its association with clinical immunity. RAMA is immunogenic during infections, and at least three epitopes within RAMA are recognized by hyperimmune sera in immunoblots. Sera from individuals living in a region of Vietnam where malaria is endemic possessed strong antibody responses toward two C-terminal regions of RAMA."

"Cytophilic antibody isotypes (immunoglobulin ...

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