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Nucleotide sequencing-based genotyping used to assess HPV disease link in Japan.
2004 AUG 5 - (NewsRx.com & NewsRx.net) -- Researchers in Japan have conducted the first large, case-control study using nucleotide sequencing-based genotyping of cervical cancer risk associated with human papillomavirus (HPV).
According to T. Asato and colleagues at the University of Ryukyus in Okinawa, "A consensus primer pair was used to amplify DNA from the L1 region of HPV by polymerase chain reaction (PCR). By PCR, 311 of 356 patients with cervical cancer and 333 of 3,249 control individuals were positive for HPV. By the direct sequencing of PCR products, nucleotide sequences of 30 genotypes were obtained."
Among the controls the researchers found a high incidence of HPV type 52 and a low incidence of HPV 16.
"Odds ratios were estimated for 18 genotypes. Types 71, 90 and 91, previously uncharacterized, were classified as low-risk genotypes, which is consistent with predictions made on the basis of phylogeny," ...
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Source: HighBeam Research, Nucleotide sequencing-based genotyping used to assess HPV disease...