Effect of high-n-6 fat diet on gene expression determined.

2004 AUG 5 - (NewsRx.com & NewsRx.net) -- Genes differentially expressed during a carcinogenic high-n-6 fat diet have been identified using microarray analysis.

"In previous studies," oncologists in Spain "demonstrated that high corn oil diets promote the development of 7,12-dimethylbenz(alpha)anthracene (DMBA)-induced mammary tumors."

In their subsequent study, E. Escrich and coauthors at the Autonomous University of Barcelona "investigated whether modulation of gene expression is one of the mechanisms by which this high-fat diet exerts such effects."

"Female Sprague-Dawley rats were induced with DMBA and fed normolipidic (3% corn oil) or high-fat (20% corn oil) diet," the scientists wrote in the journal Molecular Carcinogenesis. "Screening of genes differentially expressed in adenocarcinomas from the high corn oil diet group compared to the control diet group was performed with cDNA microarrays. The resulting six upregulated and nine downregulated genes were validated by Northern blot and/or reverse transcription (RT)-polymerase chain reaction (PCR)."

"Further investigation in a higher number of adenocarcinomas showed that in the high-fat n-6 diet group, where the tumor phenotype was verified to be more aggressive, the expression of submaxillary gland alpha-2u globulin, vitamin D[subscript]3-upregulated protein 1 (VDUP1), H19, and the unknown function gene that codifies the expressed ...