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2004 AUG 5 - (NewsRx.com & NewsRx.net) -- A study comparing agreement between nuclear magnetic resonance (NMR) and gradient gel electrophoresis (GGE) for measuring low-density lipoprotein (LDL) particle size found the two techniques are not necessarily interchangeable in women and patients with diabetes.
D.R. Witte and colleagues at the Royal Free & University College of London "measured LDL size by NMR and GGE in 324 individuals (152 with type 1 diabetes and 172 controls)."
They reported, "The Spearman correlation between both methods was 0.39 [95% confidence interval (CI)=0.29, 0.48]. The average difference was 5.38 nm (NMR being smaller), but it increased with increasing LDL size. Less than 50% of people classified as pattern B on GGE were classified as pattern B on NMR (K=0.31; 95% CI=0.17, 0.45)."
Their analysis revealed lower agreement "for diabetic subjects compared with controls, for women compared with men and for subjects with triglycerides <1.30 mmol/L compared with subjects with triglycerides>1.30 mmol/L."
"External validation showed that cholesteryl ester transfer rate was related to LDL size on GGE in all subgroups and to LDL size on NMR only in men and nondiabetic subjects," the researchers said.
They noted, therefore, that ...