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A new quantitation assay details human papillomavirus load.
2004 JUL 1 - (NewsRx.com & NewsRx.net) -- A new quantitation assay details human papillomavirus load.
"Infection with mucosotropic human papillomavirus (HPV) is the necessary cause of cervical intraepithelial neoplasia. Several epidemiological studies suggest that HPV viral load can be a risk factor of cervical dysplasia. The purpose of the present study was to evaluate a methodology to determine HPV viral load of eight oncogenic HPV types (16, 18, 31, 39, 45, 51, 52, and 58)," scientists writing in the Journal of Molecular Diagnostics report.
"The quantitation assay is based on a high-throughput real-time polymerase chain reaction (PCR). The E6-E7 region of HPV types 16, 18, 45, and 51 were used to amplify specific DNA sequences and cloned into a plasmid vector. The constructs for HPV types 16, 18, 45, and 51, and the whole genome for HPV types 31, 39, 52, and 58 were quantitated using a limiting dilution analysis and used to create standard curves. Type-specific HPV clones were used to determine specificity, linearity, and intra- and inter-assay variation," said R. Flores-Munguia and colleagues, University of Arizona, Arizona Cancer Center.
"The sensitivity of our assay covered a dynamic range of up to 7 orders of magnitude with a ...
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...vulvar and vaginal dysplasias caused by HPV types 16 and 18, two types strongly associated...observed in women who were not infected with HPV types targeted by the vaccine -- 6, 11...GARDASIL helps to protect against the four HPV types known to cause genital warts and low...
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...detect multiple human papillomavirus (HPV) types and multiple HPV infections in cervical...pairs with homology to most known genital HPV types were tested, and the L1C1-L1C2M pair...control patterns, consisting of those for HPV types 16, 18, 31, 33, 39, 45, 51, 52...
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- Press release article from: Business Wire October 6, 2005 700+ words
...associated with human papillomavirus (HPV) types 16 and 18 in a new phase III study...CIN 2/3 or AIS) associated with HPV types 16 and 18 (p less than 0.001); no...2/CIN 3, or AIS) associated with HPV types 16 and 18 by 97 percent (n=5,736...
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- Magazine article from: OB GYN News Mechcatie, Elizabeth April 1, 2009 700+ words
...the first DNA-based test for these HPV types, according to a written statement issued by the FDA. HPV types 16 and 18 cause of about 70% of cervical...essentially all of the high-risk HPV types in cervical cell samples." Both tests...
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- Newspaper article from: Cancer Vaccine Week November 24, 2008 700+ words
...and 45 (see also Qiagen). About 30 HPV types are spread through genital contact...well. "Most of the focus has been on HPV types 16 and 18, because they are the most prevalent carcinogenic HPV types and are targeted by the new HPV vaccines...
Source: HighBeam Research, A new quantitation assay details human papillomavirus load.