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Assay to quantify antibodies to Neisseria meningitidis developed.
2004 JUN 2 - (NewsRx.com & NewsRx.net) -- Scientists have developed a tetraplex flow cytometric assay for quantitation of serum antibodies to Neisseria meningitidis serogroups A, C, Y, and W-135.
According to a study from England, "A rapid and simple method for the simultaneous quantitation of serum immunoglobulin G (IgG) antibodies specific for Neisseria meningitidis serogroups A, C, Y, and W-135 was developed and evaluated. Four bead sets were generated, each conjugated with one of the meningococcal capsular polysaccharides (A, C, Y, or W-135) and serologically assessed by the use of antimeningococcal international reference sera. Cross-reactivity studies demonstrated no inhibition between monoplex and multiplex assays, and the assay was linear over a 24-fold serum dilution range.
"Inhibition studies demonstrated that the assay is specific, with
The researchers concluded, "The tetraplex assay has the potential to be an important addition to the serologic evaluation of meningococcal capsular polysaccharide conjugate vaccines."
Lal and colleagues published the results of their research in Clinical and Diagnostic Laboratory Immunology (Development and evaluation of a tetraplex flow cytometric assay for quantitation of serum antibodies to Neisseria meningitidis serogroups A, C, Y, ...
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Source: HighBeam Research, Assay to quantify antibodies to Neisseria meningitidis developed.