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2004 JUN 2 - (NewsRx.com & NewsRx.net) -- Circulating type 1 vaccine-derived poliovirus was isolated from sewage and stream waters in Hispaniola.
"Twenty-one cases of acute flaccid paralysis (AFP) were reported on the island of Hispaniola in 2000. Laboratory analysis confirmed the presence of circulating vaccine-derived poliovirus (cVDPV) type 1 in stool samples obtained from patients. As a complement to the active search for cases of AFP, environmental sampling was conducted during November and December 2000, to test for cVDPV in sewage, streams, canals, and public latrines," investigators in the United States and England report.
"Fifty-five environmental samples were obtained and analyzed for the presence of polioviruses by use of cell culture followed by neutralization and reverse-transcription polymerase chain reaction," said Jan Vinje at the University of North Carolina and collaborators. "Of the 23 positive samples, 10 tested positive for poliovirus type 1, 7 tested positive for poliovirus type 2, 5 tested positive for poliovirus type 3, and 1 tested positive for both poliovirus type 2 and type 3.
"By sequence analysis of the complete viral capsid gene 1 (VP1), a 2.1%-3.7% genetic sequence difference between 7 type 1 strains and Sabin type 1 vaccine ...
Source: HighBeam Research, Vaccine-derived poliovirus isolated from stream waters in Hispaniola.