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Dendritic cell vaccine efficacy quantified using flow cytometry.
2004 JUN 2 - (NewsRx.com & NewsRx.net) -- The protective efficacy of dendritic cell based vaccines in a human papillomavirus type 16 murine challenge model was quantified using flow cytometry.
According to recent research published in the Journal of Virological Methods, "murine model for the assessment of protective immunity to human papillomavirus (HPV) type 16, a virus that does not naturally infect mice, is described. In this system, protection was tested following intranasal challenge of mice with a recombinant vaccinia virus expressing both the selected HPV antigen and a beta-galactosidase (beta-gal) reporter. The extent of viral infectivity was determined by measuring beta-gal positive lung cells using flow cytometry.
"The efficacy of this model to measure protective immunity was evaluated by priming mice with the beta-gal vaccinia virus then challenging the mice with the same virus," said Lucy Heinemann and collaborators at the University of Otago and Wellington School of Medicine in New Zealand. "Vaccinia primed mice had negligible numbers of beta-gal positive cells in the lung 5 days following viral challenge indicating protection, whereas around 50% of cells were infected in immunologically naive, challenged mice. The protective efficacy of two dendritic cell ...
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