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DNA dry powder for non-viral gene delivery prepared by freeze-drying.
2004 JUN 2 - (NewsRx.com & NewsRx.net) -- DNA dry powder for non-viral gene delivery can be prepared by spray-freeze drying.
According to a study from Taiwan, "This study investigates the feasibility of using the process of spray-freeze drying (SFD) to produce DNA dry powders for non-viral gene delivery. The effect of protective agents was assessed on the stability of DNA dry powders after SFD. The process of SFD had adverse effects on the tertiary structure of DNA with the protective agents of sucrose, trehalose and mannitol. With the protection of these sugars, a band corresponding to the linear form of DNA was observed during gel electrophoresis between the supercoiled form (SC) and the open circular (OC) form.
"On the contrary, excess cationic condensing polyethyleneimine (PEI), in conjunction with the above sugars, had the ability to provide protection for DNA from degradation after SFD," stated Jung-hua Steven Kuo and Robin Hwang at Chia Nan University. "This is indicated by the reservation in SC and OC forms of DNA during agarose gel electrophoresis. The electrostatic forces between PEI polymer and DNA are critical for providing protection against various stresses generated by the process of SFD.
"Furthermore, on rehydration, the particle size and zeta potential of PEI/DNA complexes at weight ratios 3:1 of SFD dry powders were ...
- DNA dry powder for non-viral gene delivery prepared by freeze-drying.
- Newspaper article from: Health & Medicine Week May 31, 2004 700+ words
...NewsRx.net) -- DNA dry powder for non-viral gene delivery can be prepared...SFD) to produce DNA dry powders for non-viral gene delivery. The effect...Pharmacology (Preparation of DNA dry powder for non-viral gene delivery by spray...
- ALTERNATIVE TO VIRAL GENE THERAPY DEMONSTRATES PRE-CLINICAL SUCCESS
- Press release article from: PR Newswire November 10, 1994 700+ words
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- Chitosan augments the efficacy of non-viral gene transfer.
- Newspaper article from: Gene Therapy Weekly May 20, 2004 700+ words
...Chitosan augments the efficacy of non-viral gene transfer. "Currently, the major drawback...in gene therapy are based on viral or non-viral gene delivery systems. The viral gene delivery system shows a high transfection yield...
- Cell Genesys issued patent for adeno-associated viral gene therapy.(Brief...
- Newspaper article from: BIOTECH Patent News May 1, 2000 700+ words
...compositions and methods of adeno-associated viral gene delivery technology. Cell Genesys...property position in the adeno-associated viral gene therapy area," stated Stephen A. Sherwin...positive results using adeno-associated viral gene therapy in animal studies for hemophilia...
- MaxCyte and Northern Therapeutics Collaborate to Develop Products for Pulmonary...
- Press release article from: Business Wire February 4, 2003 700+ words
...license agreement to develop multiple non-viral gene-based products to treat pulmonary diseases...is playing a key role in making non-viral gene therapy the preferred therapeutic option...provides Northern Therapeutics with a non-viral gene delivery platform to realize the full...
- MaxCyte and Cognate Therapeutics Collaborate to Develop Products for Oncology;...
- Press release article from: Business Wire July 16, 2003 700+ words
...which Cognate will develop multiple non-viral gene-based products to treat cancer utilizing...MaxCyte. "MaxCyte's expertise in non-viral gene delivery is accelerating development...Cognate Therapeutics has access to a non-viral gene delivery platform for its therapeutic...
- Viral gene transfer technologies depict the transcriptional response to hypoxia.
- Newspaper article from: Angiogenesis Weekly February 27, 2004 700+ words
2004 FEB 27 - (NewsRx.com & NewsRx.net) -- Viral gene transfer technologies depict the transcriptional response...analyses by overexpressing disease-implicated genes using viral gene transfer methodologies," researchers advised. White and...
- Viral gene therapy shows promise for treating sarcoma.
- Newspaper article from: Biotech Business Week February 9, 2004 700+ words
2004 FEB 9 - (NewsRx.com & NewsRx.net) -- Viral gene therapy shows promise for treating sarcoma. According to...Opinion on Biological Therapy (Prospects and RISC score of viral gene therapy for sarcoma. Expert Opin Biol Ther, 2003;3(8...
- Viral gene transfer of the antiapoptotic factor Bcl-2 is protective.
- Newspaper article from: Heart Disease Weekly December 15, 2002 700+ words
...who conducted their study "to assess the effect of in vivo viral gene transfer of the antiapoptotic factor Bcl-2 to block apoptosis...Chatterjee and colleagues published their study in Circulation (Viral gene transfer of the antiapoptotic factor Bcl-2 protects against...
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- Magazine article from: Science News Travis, John January 25, 1997 700+ words
...of Allergy and Infectious Diseases in Bethesda, Md. The viral gene under suspicion encodes a protein that strongly resembles...Gershengorn. Researchers caution that the case against the viral gene remains circumstan- tial. Current tests, such as those...
Source: HighBeam Research, DNA dry powder for non-viral gene delivery prepared by freeze-drying.