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Phosphorylated HSP27 essential for association of RhoA with PKC alpha.
2004 MAY 14 - (NewsRx.com & NewsRx.net) -- Phosphorylated HSP27 is essential for acetylcholine-induced association of RhoA with PKC alpha.
According to a study from the United States, "Reorganization of the cytoskeleton and association of contractile proteins are important steps in modulating smooth muscle contraction. Heat shock protein (HSP) 27 has significant effects on actin cytoskeletal reorganization during smooth muscle contraction. We investigated the role of phosphorylated HSP27 in modulating acetylcholine-induced sustained contraction of smooth muscle cells from the rabbit colon by transfecting smooth muscle cells with phosphomimic (3D) or nonphosphomimic (3G) HSP27.
"In 3G cells, the initial peak contractile response at 30 seconds was inhibited by 25% (24.0[+ or -]4.5% decrease in cell length, n=4)," reported Suresh B. Patil and colleagues at the University of Michigan. "The sustained contraction was greatly inhibited by 75% [9.3[+ or -]0.9% decreases in cell length (n=4)]. Furthermore, in 3D cells, translocation of both PKCalpha and of RhoA was greatly enhanced and resulted in a greater association of PKCalpha-RhoA in the membrane fraction. In 3G transfected cells, PKCalpha and RhoA failed to translocate in response to stimulation with acetylcholine, resulting in an inhibition of association of PKCalpha-RhoA in the membrane fraction.
- Effect of chronic portal hypertension on RhoA/Rho-kinase mediated vascular...
- Magazine article from: Proceedings of the North Dakota Academy of Science Zhang, Hai-Ying Pavlish, Kristin M. Chen, Xuesong Machado-Arando, David Anderson, Cindy M. Dean, David A. Benoit, Joseph N. April 1, 2004 700+ words
...portosystemic shunting on RhoA/Rho-kinase mediated vascular smooth muscle signaling pathway...suggest the target of RhoA/Rho-kinase pathway...circulation alters vascular smooth muscle contraction related...down-regulated RhoA/Rho-kinase pathway...
- Phosphorylated HSP27 essential for association of RhoA with PKC alpha.
- Newspaper article from: Gastroenterology Week May 10, 2004 700+ words
...association of RhoA with PKC alpha...steps in modulating smooth muscle contraction. Heat...contraction of smooth muscle cells from the...by transfecting smooth muscle cells with phosphomimic...PKCalpha and of RhoA was greatly enhanced...
- Raf and RhoA cooperate to transform intestinal epithelial cells.
- Newspaper article from: Proteomics Weekly August 2, 2004 700+ words
...com & NewsRx.net) -- Raf and RhoA cooperate to transform intestinal epithelial...interactions between Raf and the small GTPase RhoA were studied in RIE-1 cells overexpressing both activated Raf(22W) and activated RhoA(63L)," said Jianguo Du and colleagues...
- RhoA and zeta PKC control different modes of chemokine-induced LFA-1 activation.
- Newspaper article from: Immunotherapy Weekly July 21, 2004 700+ words
...NewsRx.com & NewsRx.net) -- RhoA and zeta PKC control different modes of...of Verona found that "the small GTPase RhoA and the atypical zeta PKC differently control...state and lateral mobility is controlled by RhoA through the activity of distinct effector...
- RhoA stimulates signals conductive to endothelial cell invasion.
- Newspaper article from: Drug Week February 13, 2004 700+ words
...NewsRx.com & NewsRx.net) -- RhoA stimulates signals conductive to endothelial...poorly defined. Here we report that small RhoA GTPase is implicated in the invasion of...1). Ectopic expression of active-RhoA GTPase induced the expression of matrix...
- Akt and RhoA may have therapeutic value for diabetic cardiomyopathy.
- Newspaper article from: Cardiovascular Week March 10, 2003 700+ words
...com & NewsRx.net) -- Akt and RhoA may provide therapeutic value for the treatment...kinase Akt were assessed by Western blot. RhoA and IGF-I/IGF-I receptor mRNA levels...activation were reduced, whereas PLB and RhoA were enhanced in OVE26 hearts. GLUT4 was...
- RNA editing of serotonin receptor disrupts transactivation of G-protein RhoA.
- Newspaper article from: Health & Medicine Week March 8, 2004 700+ words
...transactivation of the small G-protein RhoA. "The human serotonin 5-HT2C receptor...transactivation of the small G-protein RhoA is required for phospholipase D activation...In contrast, neither transactivation of RhoA nor phospholipase D activation was detected...
- [Ca.sup.2+] sensitivity of smooth muscle and nonmuscle myosin II: modulated by...
- Magazine article from: Physiological Reviews Somlyo, Andrew P. Somlyo, Avril V. October 1, 2003 700+ words
...agonists and trimeric and monomeric (RhoA) G proteins and guanine nucleotide...Phosphorylation (at Ser-19) of RLC of smooth muscle (reviewed in Ref. 181) and nonmuscle...receptors (171). MLCK and the RhoA-associated Rho-ki
- Acquired smooth muscle hamartoma.(Net Case)
- Magazine article from: Indian Journal of Dermatology, Venereology and Leprology Bari, Arfan Ul Rahman, Simeen Ber March 1, 2006 700+ words
...Arfan ul. Bari, Simeen Ber. Rahman Smooth muscle hamartoma is an uncommon, usually...shoulder. The diagnosis of acquired smooth muscle hamartoma was confirmed on histopathology...and was not advised any treatment. A smooth muscle hamartoma is a rare benign proliferation...
- Mechanisms of smooth muscle contraction.
- Magazine article from: Physiological Reviews Horowitz, Arie Menice, Constance B. Laporte, Regent Morgan, Kathleen G. October 1, 1996 700+ words
...current thinking on the mechanisms of smooth muscle contraction. Discussion is confined...performed in contractile differentiated smooth muscle, given that upon tissue culture, smooth muscle rapidly changes its phenotype to a noncontractile...
Source: HighBeam Research, Phosphorylated HSP27 essential for association of RhoA with PKC alpha.