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Modification of cholera toxin B subunit enhances expression in plants.
2004 MAY 5 - (NewsRx.com & NewsRx.net) -- Modification of the cholera toxin B subunit coding sequence enhanced its expression in plants.
"The cholera toxin B subunit (CTB) contains five identical polypeptides and targets glycosphingolipid receptors on eukaryotic cell surfaces. Increased expression of CTB in plants is critical for the development of edible vaccines. In this study, the coding sequence of the CTB gene was optimized, based on the modification of codon usage to that of tobacco plant genes and the removal of mRNA-destabilizing sequences," scientists in South Korea report.
"The synthetic CTB gene was cloned into a plant expression vector and expressed in tobacco plants under the control of the CaMV 35S promoter," said Tae-Jin Kang and colleagues at Chonbuk National University. "The recombinant CTB protein constituted approximately 1.5% of the total soluble protein in transgenic tobacco leaves. This level of CTB production was approximately 15-fold higher than that in tobacco plants that were transformed with the bacterial CTB gene. The recombinant CTB produced by tobacco plants demonstrated strong affinity for GM1-ganglioside, which ...
- Cholera toxin protects against Clostridium difficile toxin A.
- Newspaper article from: Vaccine Weekly January 14, 2004 700+ words
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- Peptide extensions simply, effectively produce detoxified CT...
- Newspaper article from: Vaccine Weekly December 4, 2002 700+ words
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- Group B streptococcal protein rib and cholera toxin protect against infection.
- Newspaper article from: Vaccine Weekly April 21, 2004 700+ words
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- Cholera toxin B subunit may not be helpful as adjuvant in pertussis vaccine.
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- Cholera toxin induces migration of dendritic cells.(Brief Article)
- Newspaper article from: Vaccine Weekly March 5, 2003 700+ words
...uptake of the mucosal adjuvant cholera toxin (CT) or live Salmonella bacteria...study in Infection and Immunity (Cholera toxin induces migration of dendritic...This article was prepared by Vaccine Weekly editors from staff and other...
- Cholera toxin fights autoimmune disease.
- Magazine article from: Science News Raloff, Janet April 22, 1995 700+ words
...begun harnessing a fragment of the cholera toxin in a novel approach to thwarting autoimmune...recognized as foreign by attaching it to a cholera toxin fragment. When fed to animals, a...Of the two primary components of cholera toxin, only the A chain is actually poisonous...
Source: HighBeam Research, Modification of cholera toxin B subunit enhances expression in plants.