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Response to plasmid DNAs encoding SARS-CoV spike gene fragments characterized.
2004 MAY 5 - (NewsRx.com & NewsRx.net) -- Researchers have characterized the humoral responses in mice immunized with plasmid DNAs encoding SARS-CoV spike gene fragments.
"The immunological characteristics of SARS-CoV spike protein were investigated by administering mice with plasmids encoding various S gene fragments. We showed that the secreting forms of S1, S2 subunits and the N-terminus of S1 subunit (residues 18-495) were capable of eliciting SARS-CoV specific antibodies and the region immediate to N-terminus of matured S1 protein contained an important immunogenic determinant for elicitation of SARS-CoV specific antibodies. In addition, mice immunized with plasmids encoding S I fragment developed a Th1-mediated antibody isotype switching," investigators in China report.
"Another interesting finding was that mouse antibodies elicited separately by plasmids encoding S1 and S2 subunits cooperatively neutralized SARS-CoV but neither the S1 nor S2 specific antibodies did, suggesting the possible role of both S1 and S2 subunits in host cell docking and entry," stated Fanya Zeng and colleagues at the University of Hong Kong. ...
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