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Neuraminidase-deficient influenza virus is potential vaccine vector.
2004 MAY 5 - (NewsRx.com & NewsRx.net) -- Scientists have characterized a neuraminidase-deficient influenza A virus that is a potential gene delivery vector and a live vaccine.
"We recently identified a packaging signal in the neuraminidase (NA) viral RNA (vRNA) segment of an influenza A virus, allowing us to produce a mutant virus [GFP(NA)-Flu] that lacks most of the NA open reading frame but contains instead the gene encoding green fluorescent protein (GFP). To exploit the expanding knowledge of vRNA packaging signals to establish influenza virus vectors for the expression of foreign genes, we studied the replicative properties of this virus in cell culture and mice," scientists in Japan report.
"Compared to wild-type virus, GFP(NA)-Flu was highly attenuated in normal cultured cells but was able to grow to a titer of >10[superscript]6 PFU/mL in a mutant cell line expressing reduced levels of sialic acid on the cell surface," said Kyoko Shinya at the University of Tokyo and collaborators throughout Japan. "GFP expression from this virus was stable even after five passages in the latter cells. In intranasally infected mice, GFP was detected in the epithelial cells of nasal mucosa, bronchioles, and alveoli for up to 4 days postinfection.
"We attribute the attenuated growth of GFP(NA)-Flu to virion aggregation at the surface of bronchiolar epithelia," stated the researchers. "In studies ...
- Neuraminidase-deficient influenza virus is potential vaccine vector.
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Source: HighBeam Research, Neuraminidase-deficient influenza virus is potential vaccine vector.