AccessMyLibrary provides FREE access to over 30 million articles from top publications available through your library.
Create a link to this page
Copy and paste this link tag into your Web page or blog:
gONC increases conformational stability and toxicity for cancer cells.
2004 MAY 5 - (NewsRx.com & NewsRx.net) -- Glycosylation of onconase increases its conformational stability and toxicity for cancer cells.
"Onconase (ONC) is currently in Phase III clinical trials as a cancer chemotherapeutic agent. Despite the finding that ONC contains an N-linked glycosylation site (-N69-V70-T71-), only the non-glycosylated form of the protein has been identified to date." The Pichia pastoris expression system was employed "to produce recombinant glycosylated ONC (gONC)protein," wrote scientists in South Korea.
"Approximately 10 mg of ONC protein was secreted per liter of culture media, of which about 80% was glycosylated at N69. CD spectroscopic analyses revealed that the secondary structure of gONC is identical to that of ONC." gONC was determined to contain "a high-mannose core structure. Importantly, glycosylation of ONC at N69 greatly increased its toxicity for K-562 cancer cells. Specifically, the IC[subscript]50 value of gONC was 50-fold lower than that of ONC," reported B.M. Kim and colleagues.
"Glycosylation increased both the T-m of ONC and ...
- gONC increases conformational stability and toxicity for cancer cells.
- Newspaper article from: Genomics & Genetics Weekly May 7, 2004 700+ words
...increases its conformational stability and toxicity for cancer cells. "Onconase...related to higher conformational stability," researchers...increases its conformational stability and toxicity for cancer cells. Biochem Biophys...
- Preparation, infrared and Raman spectra, conformational stability, ab initio...
- Magazine article from: Bulletin of the South Carolina Academy of Science Bregg, Jason Guirgis, Gamil Heldrich, Rick Durig, James January 1, 2003 700+ words
Cyclopropyl bromosilane was prepared for the first time by cyclizing trimethoxy vinyl silane with methylene iodide using the Simmons-Smith reaction. The purity of the compound was checked by mass spectroscopy and NMR spectroscopy. The infrared (3300-30[cm.sup.-1]) spectra of gaseous and solid
- Why cancer cells are addicted to sugar ... and what you can do about it.
- Newspaper article from: Women's Health Letter September 1, 2008 700+ words
...ve probably heard that sugar feeds cancer cells. This is why knowledgeable doctors...You see, like all living things, cancer cells want to stay alive, and eating sugar...tumors. There's another reason why cancer cells are addicted to sugar. Sugar keeps...
- New oncology strategy: molecular targeting of cancer cells.
- Magazine article from: Dermatology Nursing Capriotti, Teri June 1, 2005 700+ words
New understanding of how cancer cells survive, thrive, and metastasize...Describe molecular targets of cancer cells under investigation. 2. Discuss...cancer therapies which target cancer cells specifically and selectively with...
- PhytoMedical's Patented Anti-Cancer Compounds Kill Lung Cancer Cells, a...
- Press release article from: Business Wire January 22, 2008 700+ words
...are designed to bind to DNA and kill cancer cells; previously demonstrated ability to kill brain cancer cells, an extremely resistant and often...DMS 114 strain of human small lung cancer cells, an important outcome given lung cancer...
- New oncology strategy: molecular targeting of cancer cells.(Nursing...
- Magazine article from: MedSurg Nursing Capriotti, Teri June 1, 2004 700+ words
...anti-cancer therapies which target cancer cells specifically and selectively with less...chemotherapeutic agents do not target cancer cells selectively, leading to widespread...of proliferation. Therefore, both cancer cells and mitotically active healthy cells...
- Prognostic Significance of Circulating Breast Cancer Cells in Patients with...
- Newspaper article from: Clinical Oncology Alert April 1, 2008 700+ words
...Prognostic Significance of Circulating Breast Cancer Cells in Patients with Known Metastatic Disease Abstract...marrow were assayed for the detection of breast cancer cells. Breast cancer cells were found in the bone marrow in 59% of patients...
- Aggressive Cancer Cells.
- Magazine article from: Townsend Letter for Doctors and Patients Klotter, Jule August 1, 2001 700+ words
Scientists have discovered that cancer cells can protect themselves from immune cell...by actively destroying T cells. Some cancer cells, for example, do not produce antigens...that incite immune activity. Other cancer cells grow behind a screen of proteins that...
- Good versus bad cells.(cell death and metabolism of cancer cells and...
- Magazine article from: The Science Teacher February 1, 2009 700+ words
Cancer cells and nervous system neurons may not look...describe how neurons (nerve cells) and cancer cells (see image above) achieve the common...Lineberger Comprehensive Cancer Center. "In cancer cells, blocking cell death allows them to...
- HIV Protein Enlisted To Help Kill Cancer Cells.
- Magazine article from: Space Daily February 16, 2007 700+ words
...Writers ST. LOUIS, Feb. 16 (SPX) -- Cancer cells are sick, but they keep growing because...efficient way to get a messenger into cancer cells that forces them to respond to death...TAT to pull a protein called Bim into cancer cells. TAT alone cannot cause AIDS and has...
Source: HighBeam Research, gONC increases conformational stability and toxicity for cancer cells.