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Dendritic cell subsets induce antigen-specific cytotoxic T cell responses.
2004 MAY 5 - (NewsRx.com & NewsRx.net) -- Dendritic cell subsets generated from CD34+ hematopoietic progenitors can be transfected with mRNA and induce antigen-specific cytotoxic T cell responses.
"Human dendritic cells (DCs) generated in culture from either monocytes or CD34+ hematopoietic progenitor cells (CD34-HPCs) have been used in cancer immunotherapy protocols with encouraging results. Yet an optimal strategy for the delivery of antigen to DCs still remains to be established. Recent studies demonstrated the feasibility of mRNA transfection to load monocyte-derived DCs," scientists writing in the Journal of Immunological Methods report.
"It is not known, however, whether DCs derived by culturing CD34-HPC with GM-CSF and TNF-alpha for 9 days (CD34-DCs) can be efficiently transduced with mRNA," said Hideki Ueno and colleagues at the Baylor Institute for Immunology Research. "Here we show that clinical-grade CD34-DCs generated after 8 days of culture can be transfected with mRNA without significant alteration of cell viability. About 90% of cells transfected with GFP-RNA express GFP 24 hours post-transfection. Remarkably, transfected CD34-DCs retain high levels of GFP expression for at least 14 days. CD34-DCs transfected with Flu-MP RNA were highly efficient in inducing the proliferation of Flu-MP-specific CD8+ T cells as measured by tetramer staining.
"Furthermore, the ...
- Dendritic cell subsets induce antigen-specific cytotoxic T cell responses.
- Newspaper article from: Cancer Weekly May 4, 2004 700+ words
...study in the Journal of Immunological Methods (Dendritic cell subsets generated from...publisher of the Journal of Immunological Methods can be contacted at...areas of Cancer Vaccine, Dendritic Cell Vaccine, Vaccine Development...
- Centrifugation Improves Dendritic Cell Transduction Efficiency.
- Newspaper article from: Immunotherapy Weekly Heinrich, Deborah W. August 15, 2001 700+ words
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- Dendritic cell yields can be improved in culture.(Brief Article)
- Newspaper article from: Immunotherapy Weekly June 26, 2002 700+ words
...calcium signalling agents - successfully enhanced in vitro DC yields, according to their report in the Journal of Immunological Methods. The researchers first expanded cord blood and/or bone marrow-derived stem cells for 10 days using a mixture...
- Dendritic cell yields can be improved in culture.
- Newspaper article from: Blood Weekly June 27, 2002 700+ words
...calcium signalling agents - successfully enhanced in vitro DC yields, according to their report in the Journal of Immunological Methods. The researchers first expanded cord blood and/or bone marrow-derived stem cells for 10 days using a mixture...
- Aastrom Biosciences Executes Evalaution Agreements With Leading German...
- Press release article from: PR Newswire April 19, 2001 700+ words
- DC-I Dendritic Cell Product Advances Toward U.S...European evaluation of its DC-I dendritic cell product for use in cancer vaccines...School teaching hospital. "As dendritic cell-based vaccines are being broadly...
- Aastrom Biosciences Begins Site Testing New Dendritic Cell Vaccine Product for...
- Press release article from: PR Newswire March 15, 2001 700+ words
...site evaluation of Aastrom's DC-I dendritic cell product for use in vaccines to treat...external phase of a plan to make its dendritic cell product broadly available for dendritic cell-based cancer vaccines. Once the beta...
- Dendritic cell-based glioma immunotherapy reviewed.
- Newspaper article from: Cancer Vaccine Week August 18, 2003 700+ words
...amp; NewsRx.net) -- A review of dendritic cell-based glioma immunotherapy has been...Niigata University. "Therefore, dendritic cell-based immunotherapy could be a new...therapy." "A literature review of dendritic cell-based glioma immunotherapy was used...
- Aastrom Biosciences Completes Second CE Marked Dendritic Cell Therapy Product;...
- Press release article from: PR Newswire February 14, 2002 700+ words
...production of a complete antigen-loaded dendritic cell vaccine, based on Aastrom's proprietary cultured dendritic cell, the Dendricell(TM). The DCV-I joins the Company's lead dendritic cell product, the DC-I, in providing a unique...