AccessMyLibrary provides FREE access to over 30 million articles from top publications available through your library.
Create a link to this page
Copy and paste this link tag into your Web page or blog:
Methylated CpG-containing plasmid activates immune system.
2004 APR 21 - (NewsRx.com & NewsRx.net) -- Methylated CpG-containing plasmid activates the immune system.
According to published research from France, "Bacterial DNA differs from mammalian DNA by the presence of unmethylated cytosine-phosphate-guanosine (CpG) motifs. The immunostimulatory properties of a DNA vaccine have been suspected to be associated with these motifs. The aim of this study was to assess the inactivation of the immunostimulatory potential of a plasmid after methylation of its CpG motifs."
"We constructed two identical non-coding plasmids, and one of these was de novo methylated on its CG sequences," said Sylvie Cornelie and colleagues at Pasteur Institut de Lille. "A single administration of recombinant antigen with methylated or unmethylated CpG-containing plasmid was performed in mice. As expected, only unmethylated CpG-containing plasmid enhanced the specific immune response. However, a study of in vivo activation of Langerhans' cells and analysis of mRNA synthesis indicated that both the plasmids promoted cell emigration and cytokine induction."
"These data ...
- Methylated CpG-containing plasmid activates immune system.
- Newspaper article from: Biotech Week April 21, 2004 700+ words
...NewsRx.net) -- Methylated CpG-containing plasmid activates the immune...highlight that a methylated CpG-containing plasmid is not inert and...of Immunology (Methylated CpG-containing plasmid activates the immune...
- Report summarizes gastritis study findings from T. Tahara and...
- Newspaper article from: Cancer Weekly July 7, 2009 700+ words
...association between the increased number of methylated CpG islands and H. pylori infection was...0.0001). An increased number of methylated CpG islands was also associated with severity...participants. An increased number of methylated CpG islands correlated with lower PG I...
- Studies from Kyoto University update current data on DNA research.
- Newspaper article from: Biotech Week November 12, 2008 700+ words
...minor grooves of the methylation site. In contrast to fully methylated CpG sites recognized by the methyl- CpG- binding domain...is associated with the coordinated transfer of the hemi- methylated CpG site from UHRF1 to Dnmt1." Arita and colleagues published...
- Data on gene therapy reported by G. Chikh and co-researchers.(Report)
- Newspaper article from: Biotech Week July 8, 2009 700+ words
Research findings, 'Synthetic methylated CpG ODNs are potent in vivo adjuvants when delivered in liposomal...of their research in International Immunology (Synthetic methylated CpG ODNs are potent in vivo adjuvants when delivered in liposomal...
- Tumor suppressor gene inactivation during cadmium-induced malignant...
- Magazine article from: Environmental Health Perspectives Benbrahim-Tallaa, Lamia Waterland, Robert A. Dill, Anna L. Webber, Mukta M. Waalkes, Michael P. October 1, 2007 700+ words
...DNMT3b (Okano et al. 1998, 1999a). It is thought that DNMTs function in either maintenance of DNA methylation, where methylated CpG sites on one DNA strand are copied, or in de novo methylation, where both strands are initially unmethylated and methylation...
- DNA Vaccines "CpG-Rich Oligodeoxynucleotides Coadministered with Vaccine...
- Newspaper article from: Vaccine Weekly November 2, 1998 700+ words
...in newborn mice. To investigate whether this was due to prolonged in vivo antigen production or to the presence of non-methylated CpG repeats on bacterial plasmids, short oligodeoxynucleotides (ODN) containing CpG repeats were coadministered with several...
- Genetic interactions modulate heparanase expression.
- Newspaper article from: Drug Week December 19, 2003 700+ words
...heparanase promoter was associated with inactivation of the affected allele. Despite lack of sequence homology, extensively methylated CpG islands were found both in human choriocarcinoma (JAR) and rat glioma (C-6) cells which lack heparanase activity...
- Recent findings from Emory University, Medical Department highlight research in...
- Newspaper article from: Medical Devices & Surgical Technology Week November 16, 2008 700+ words
...methylation by interacting with DNA nucleotide methyltransferase 1 ( DNMT1), the maintenance methyltransferase, and with hemi-methylated CpG, the substrate for DNMT1 ( refs 1 and 2)," scientists writing in the journal Nature report (see also DNA Research...
