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Replication kinetics of Chimerivax-DEN in Aedes aegypti determined.
2004 APR 7 - (NewsRx.com & NewsRx.net) -- Replication kinetics of the Chimerivax-DEN 1, 2, 3, 4 tetravalent virus mixture in Aedes aegypti were determined using real-time reverse transcriptase-polymerase chain reaction.
According to recent research published in the American Journal of Tropical Medicine and Hygiene, "The vector competence of mosquitoes for chimeric viruses being developed as vaccines to protect against dengue (DEN) virus infection were evaluated in a cooperative agreement with Acambis, Inc. Chimeric viruses have been constructed that contain the premembrane (prM) and envelope (E) genes of each of the wild-type (wt) DEN virus serotypes, DEN-1, DEN-2, DEN-3, and DEN-4, in the yellow fever (YF) vaccine virus (strain 17D) YF-VAX backbone."
"It was previously shown that the replication profile of ChimeriVax-DEN2 virus in Aedes albopictus C6/36 cells and in vivo in Ae. aegypti mosquitoes corresponded to that of YF-VAX virus; replication was restricted in C6/36 cells, and Ae. aegypti were poorly infected via an artificial infectious blood meal," reported Barbara W. Johnson and collaborators at the U.S. Centers for Disease Control and Prevention and Acambis, Inc. "Thus, there is very little risk of transmission by mosquitoes of ChimeriVax-DEN2 vaccine virus through the bite of a mosquito."
"However, because ChimeriVax-DEN 1, 2, 3, 4 viruses will be administered to humans simultaneously, growth of a mixture of ChimeriVax-DEN 1, 2, 3, 4 viruses was assessed in both C6/36 cells in culture and in the Ae. aegypti mosquito, which is the primary vector of both YF and DEN viruses," stated Johnson and her coauthors. "Mosquitoes were intrathoracically (IT) inoculated with virus or fed a virus-laden blood meal, and the replication kinetics of ChimeriVax(TM)-DEN 1, 2, 3, 4 were compared with the wt DEN and YF-VAX viruses. A quantitative real-time reverse transcriptase-polymerase chain reaction assay was developed as a method to detect and differentiate replication of each of the four ChimeriVax-DEN serotypes in ...
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Source: HighBeam Research, Replication kinetics of Chimerivax-DEN in Aedes aegypti determined.