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Bacteriophage-mediated nucleic acid immunization offers benefits.
2004 APR 7 - (NewsRx.com & NewsRx.net) -- Bacteriophage-mediated nucleic acid immunization offers benefits over naked DNA vaccines.
"Whole bacteriophage lambda particles, containing reporter genes under the control of the cytomegalovirus promoter (P[subscript]CMV), have been used as delivery vehicles for nucleic acid immunization. Following intramuscular injection of mice with lambda-gt11 containing the gene for hepatitis B surface antigen (HBsAg), anti-HBsAg responses in excess of 150 mIU/mL were detected. When isolated peritoneal macrophages were incubated with whole lambda particles containing the gene for green fluorescent protein (GFP) under the control of P[subscript]CMV, GFP antigen was detected on the macrophage surface 8 hours later," scientists writing in the journal FEMS Immunology and Medical Microbiology report.
"Results suggested that direct targeting of antigen-presenting cells by bacteriophage 'vaccines' may occur, leading to enhanced immune responses compared to naked DNA delivery," said Jason R. Clark and John B. March at "Bacteriophage DNA vaccines offer several advantages: they do not contain ...
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