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Low-protein diet alters gene expression in rat pancreatic islets.
2004 APR 5 - (NewsRx.com & NewsRx.net) -- A low-protein diet alters gene expression in rat pancreatic islets.
"Insulin secretion is regulated mainly by circulating nutrients, particularly glucose, and is also modulated by hormonal and neuronal inputs. Nutritional alterations during fetal and early postnatal periods, induced by either low protein or energy-restricted diets, produce beta-cell dysfunction. As a consequence, insulin secretion in response to different secretagogues is reduced, as is the number of beta-cells and the size and vascularization of islets," scientists in Brazil report.
"In this study, we used a cDNA macroarray technique and RT-PCR to assess the pattern of gene expression in pancreatic islets from rats fed isocaloric low (6 g/100 g, LP) and normal (17 g/100 g, NP) protein diets, after weaning," said Viviane Delghingaro-Augusto and collaborators at the Universidade Estadual de Campinas and Universidade de Sao Paulo. "Thirty-two genes related to metabolism, neurotransmitter receptors, protein trafficking and targeting, intracellular kinase network members, and hormones had altered expression (up- or down-regulated)."
"RT-PCR confirmed the macroarray results for five selected genes, i.e., clusterin, secretogranin II precursor, eukaryotic translation initiation ...
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