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Expression of exogenous genes in Trypanosoma cruzi improved.
2004 MAR 31 - (NewsRx.com & NewsRx.net) -- Investigators have improved the expression of exogenous genes in Trypanosoma cruzi.
"To improve transfection efficiency in Trypanosoma cruzi, we developed a new electroporation protocol and expression vectors which use luciferase and green and red fluorescent proteins as reporter genes. In transient transfections, the electroporation conditions reported here resulted in luciferase expression 100 times higher than the levels obtained with previously described protocols," scientists in Brazil and Argentina report.
"To verify whether sequences containing different trans-splicing signals influence reporter gene expression, we compared DNA fragments corresponding to 5' untranslated plus intergenic (5' UTR plus Ig) regions from GAPDH, TcP2beta, alpha- and beta-tubulin and amastin genes," said Wanderson D. Darocha and collaborators at the Federal University of Minas Gerais in Brazil and the University of Buenos Aires in Argentina. "Vectors containing sequences derived from the first four genes presented similar efficiencies and resulted in luciferase expression in transiently transfected epimastigotes that was up to 10 times higher than ...
- Trypanosoma cruzi antigen and CpG-ODN protect against trypomastigotes.
- Newspaper article from: Vaccine Weekly April 28, 2004 700+ words
...A purified Trypanosoma cruzi antigen and CpG...cystein proteinase of Trypanosoma cruzi, in the process...Use of a purified Trypanosoma cruzi antigen and CpG...was prepared by Vaccine Weekly editors from staff...
- Trypanosoma cruzi proteinase protects against trypanosomiasis.
- Newspaper article from: Vaccine Weekly November 6, 2002 700+ words
...major proteinase of Trypanosoma cruzi, cruzipain, showed...protection against Trypanosoma cruzi in mice. Infect...cocultured with Trypanosoma cruzi-infected macrophages...was prepared by Vaccine Weekly editors from staff...
- Trypanosoma cruzi antigens elicit strong humoral immune response in mice.
- Newspaper article from: Vaccine Weekly December 3, 2003 700+ words
...repetitive antigen of Trypanosoma cruzi elicited a strong...antigen (FRA) of Trypanosoma cruzi. The immunization...repetitive antigen of Trypanosoma cruzi. Mem Inst Oswaldo...was prepared by Vaccine Weekly editors from staff...
- Genes from replicative form of Trypanosoma cruzi protect mice.
- Newspaper article from: Vaccine Weekly June 25, 2003 700+ words
...replicative form of Trypanosoma cruzi, the parasite responsible...by amastigotes of Trypanosoma cruzi elicits protective...identity with the Trypanosoma cruzi amastigote surface...was prepared by Vaccine Weekly editors from staff...
- Autoimmune encephalomyelitis cured by Trypanosoma cruzi infection.
- Newspaper article from: Vaccine Weekly December 1, 2004 700+ words
...cured by infection with Trypanosoma cruzi. "Trypanosoma cruzi is an intracellular parasite...cured by infection with Trypanosoma cruzi. J Autoimmun, 2004;23...article was prepared by Vaccine Weekly editors from staff and other...
- Autoimmune response induced by Trypanosoma cruzi proteins.
- Newspaper article from: Vaccine Weekly August 11, 2004 700+ words
...induced by immunization with Trypanosoma cruzi proteins. According to...from the United States, "Trypanosoma cruzi is the protozoan parasite...induced by immunization with Trypanosoma cruzi proteins. Infec Immunity...article was prepared by Vaccine Weekly editors from ...
- Tomato parasite shares antigens with Trypanosoma cruzi.
- Newspaper article from: Vaccine Weekly February 4, 2004 700+ words
...shares antigens with Trypanosoma cruzi that are recognized...reactivity between Trypanosoma cruzi, the protozoan...shares antigens with Trypanosoma cruzi that are recognized...was prepared by Vaccine Weekly editors from staff...
- Role of CD8+ T cells in Trypanosoma cruzi infection reviewed.
- Newspaper article from: Vaccine Weekly November 17, 2004 700+ words
...function of CD8+ T cells in Trypanosoma cruzi infection in a recent issue...crucial to the control of Trypanosoma cruzi infection and probably...function of CD8+ T cells in Trypanosoma cruzi infection. Immunol Rev...article was prepared by Vaccine Weekly editors from ...