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Acetyl and phosphate groups quantified in vaccine polysaccharides.
2004 MAR 31 - (NewsRx.com & NewsRx.net) -- Scientists have quantified O-acetyl, N-acetyl and phosphate groups in bacterial vaccine polysaccharides by high-performance anion-exchange chromatography with conductivity detection (HPAEC-CD).
According to published research from the United States, "The O-acetyl groups in meningococcal A and typhoid Vi polysaccharides (PSs) are functional immunogenic epitopes in humans. To quantify and determine the extent of O-acetylation in these and other bacterial vaccine PSs, anion-exchange HPLC methods have been developed for quantification of O-acetyl, N-acetyl, and phosphate groups in the PSs after these groups were hydrolyzed into anions. The O-acetylation in meningococcal A, C, Y, and W-135, pneumococcal 9V and 18C and typhoid Vi PSs were analyzed."
"The O-acetyl group was selectively released from a PS as acetate by mild alkaline hydrolysis in 10 or 20 mM NaOH at 37 degrees C until maximum release," reported George Kao and Chao-Ming Tsai at the U.S. Food and Drug Administration. "The acetate in the hydrolyzate was then quantified by high-performance anion-exchange chromatography with conductivity detection (HPAEC-CD) after removal of the PS by filtration with a 10,000 molecular-weight-cut-off membrane."
"Since the extent of O-acetylation on the PSs depends on bacterial species, strains and growth conditions, the N-acetyl group of amino-sugars, phosphate, or monosaccharide components of the PSs were also quantified using HPAEC with conductivity or amperometry detection to determine the molar ratios of the O-acetyl group to these components," said Kao and Tsai. "The average numbers of ...
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Source: HighBeam Research, Acetyl and phosphate groups quantified in vaccine polysaccharides.