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CHO cell clones expressing hepatitis B surface protein screened.
2004 FEB 11 - (NewsRx.com & NewsRx.net) -- Scientists have developed a screening method for Chinese hamster ovary (CHO) cell clones expressing histidine-tagged major S hepatitis B surface protein using a semi-quantitative PCR protocol.
According to a study from Brazil, "Different mammalian cells have been used successfully for the expression of the hepatitis B surface antigen (HBsAg). The patterns of expression of the HBsAg seem to depend on the cell type and the number of gene copies integrated into cellular genome. The expression of an HBsAg fused to histidine tag (His-HBsAg), had not been reported in mammalian cells."
"This paper describes a semi-quantitative polymerase chain reaction (PCR) employed to investigate the patterns of expression of His-HBsAg in stably transfected Chinese hamster ovary (CHO) cell clones," said Carlos Otavio Alves Vianna and collaborators at Bio-Manguinhos. "pcDNA4CR20, a mammalian expressing vector, encoding His-HBsAg, was constructed. The correlation between His-HBsAg expression and the number of integrated copies of the HBsAg gene into cell clones genome was evaluated by the semi-quantitative PCR, with limit dilutions of the genomic DNA as template."
"The results show a positive correlation between ...
- HBsAg Positive Blood Donors Followed for Hepatitis.
- Newspaper article from: Blood Weekly August 10, 2000 700+ words
...hepatitis B surface antigen postive (HBsAg) blood donors should be followed-up...conducted a study of 480 adults found to be HBsAG postive during screening of blood donors...their presentation was "Evaluation of HBsAg positive blood donors and results of long...
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- Newspaper article from: Blood Weekly Boyles, Salynn September 6, 1999 700+ words
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- Prevalence of Maternal Hepatitis B Surface Antigen (HBsAg) in Detroit,...
- Newspaper article from: Hepatitis Weekly October 11, 1999 700+ words
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- Newspaper article from: Vaccine Weekly October 11, 1999 700+ words
...Maternal Hepatitis B Surface Antigen (HBsAg) in Detroit, Hartford, New Haven, Dallas...national estimate of the annual number of HBsAg-positive women giving birth was 22...specific national natality data and limited HBsAg prevalence data from the second National...
- HBSAG RECIPIENTS HAVE POOR ANTIGEN-SPECIFIC RESPONSES.(Brief...
- Newspaper article from: Vaccine Weekly November 23, 1998 700+ words
A majority of HBsAg vaccine recipients show good anti-HBs...0, 1 and 6 months) of plasma derived HBsAg vaccine ("Lack of in vitro Lymphoproliferative...to show lymphoproliferative responses to HBsAg in spite of having a very good anti-HBs...
- HBV DNA test should replace HBsAg and/or anti-HBc for blood donor screening.
- Newspaper article from: Blood Weekly June 10, 2004 700+ words
...hepatitis B virus DNA test should replace HBsAg and/or anti-HBc for blood donor screening...donors using progressively more sensitive HBsAg assays; in some countries anti-HBc assays...low-level viremia who lack detectable HbsAg," wrote scientists at the University...