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A-beta single chain Fv antibodies isolated from immune phage library.
2004 FEB 11 - (NewsRx.com & NewsRx.net) -- Amyloid-beta peptide-specific single chain Fv antibodies have been isolated from an immune phage display library.
According to recent research published in the Journal of Neuroimmunology, "A single-chain fragment variable (scFv) antibody library displayed on phage was constructed using spleen cells from mice immunized with human amyloid-beta peptide (A-beta42). This first anti-A-beta42 scFv immune antibody library was selected against human A-beta42. A number of positive clones were obtained, and sequences of V[subscript]H and V[subscript]kappa genes were analyzed using ExPASy and BLAST computer tools."
"This analysis revealed that only two unique clones with identical V[subscript]H and V[subscript]kappa complementarity determining region (CDR) (except HCDR2) and identical germline genes were selected, indicating that oligoclonal immune response was occurring in A-beta42-immunized mice," reported Karen Manoutcharian and colleagues at the Universidad Nacional Autonoma de Mexico and the University of South Florida in the U.S. ...
- A-beta single chain Fv antibodies isolated from immune phage library.
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