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A combination of estriol and vitamin D-3 keeps bone-forming cells viable.
2004 FEB 5 - (NewsRx.com & NewsRx.net) -- A combination of estriol and vitamin D-3 keeps bone-forming cells viable.
According to published research from Japan, "it is clinically evident that administration of estriol (E-3) increases the bone mass density of the lumbar vertebrae in postmenopausal women, and that combined treatment with estrogen and 1,25-dihydroxyvitamin D-3 (VD3) increases femoral neck bone mass density compared with treatment with estrogen alone in postmenopausal osteoporotic women. However, the molecular mechanism whereby treatment with E-3 affects osteoblast cell function is still unknown. This study was conducted first to examine the comparative effects of E-3 and VD3 on the cell viability of cultured human osteoblast-like cells (HOS) and second to determine whether E-3 affects VD3 receptor mRNA expression in HOS."
"The cell viability and VD3 receptor mRNA expression of cultured HOS were assessed by MTT assay and semi-quantitative reverse transcriptase-polymerase chain reaction with Southern blot analysis, respectively. The treatment with E-3 increased the cell viability Of cultured HOS compared with untreated control cultures," Y. Yamanaka and colleagues, Kobe University, Graduate School of Medicine reported.
"The increase in cell viability caused by the treatment with E-3 was further augmented by the combined treatment with VD3. The addition of either E-3 (3.52 x 10[superscript]-8 mol/l) or E-3 ...
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Source: HighBeam Research, A combination of estriol and vitamin D-3 keeps bone-forming cells...