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Pan-pestivirus PCR primers cross-react with poliovirus genome.
2004 FEB 4 - (NewsRx.com & NewsRx.net) -- Researchers report that pan-pestivirus PCR primers cross-react with the poliovirus genome.
According to recent research from Slovakia and the U.K., "When several human vaccines were tested for pestivirus contamination using a one-tube closed nested RT-PCR method employing pan-pestivirus primers selected from the 5'-untranslated region (5'-UTR) of the pestivirus genome, a 224-bp DNA product was produced from a poliovirus vaccine. Although this amplicon was of the size expected for pestiviruses, its sequence showed a 100% similarity with the corresponding reverse complement of a nucleotide sequence from the VP2 gene of the poliovirus type I Sabin strain."
"It is recommended that all positive PCR products, especially those prepared using pan-pestivirus primers, obtained from screening biological substances for pestivirus contamination should be checked by use of a specific hybridization probe and preferably by ...
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Source: HighBeam Research, Pan-pestivirus PCR primers cross-react with poliovirus genome.