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Dengue 4 virus glycoprotein induces resistance to Dengue 4 virus.
2004 JAN 7 - (NewsRx.com & NewsRx.net) -- The carboxy-terminally truncated Dengue 4 virus envelope glycoprotein expressed in Pichia pastoris induced neutralizing antibodies and resistance to Dengue 4 virus challenge in mice.
"We have expressed a recombinant Dengue 4 virus envelope glycoprotein (E4rec), truncated at its C-terminus by 53 amino acids, in Pichia pastoris. The presence of E4rec was confirmed by Western-blot using anti-DEN 4 hyper immune mouse ascitic fluid. E4rec migrated during SDS-PAGE as a 64 kDa protein," researchers in Cuba report.
"Treatment with endoglycosidases showed that the E protein was modified by the addition of short mannose chains and the absence of hyperglycosylation," said Mayra Mune and collaborators at the Pedro Kouri Tropical Medicine Institute and the Center of Genetic Engineering and Biotechnology in Havana. "When administered to BALB-C mice, E4rec elicited a DEN 4 neutralizing antibody response hemagglutination inhibition antibodies and specific memory T cell response. Mice immunized were also significantly ...
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Source: HighBeam Research, Dengue 4 virus glycoprotein induces resistance to Dengue 4 virus.