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Cells can be frozen with minimal loss using high-pressure freezing, planchettes.
2003 DEC 10 - (NewsRx.com & NewsRx.net) -- Cells can be frozen with minimal loss using high-pressure freezing, planchettes; even parietal and gastric gland cells remain responsive to histamine.
"We describe a procedure for high-pressure freezing (HPF) of cultured cells using HPF aluminium planchettes as a substrate," scientists writing in the Journal of Microscopy report.
"Cells are either grown directly on planchettes covered with Matrigel or allowed to attach to poly-L-lysine-coated planchettes. This method allows for rapid transfer of the cells into the HPF, and minimizes physical and physiological trauma to the cells. Furthermore, the yield of well-frozen cells approaches 100% for every cell type we have tried so far.
In this report, we show well-preserved ultrastructure in mitotic and interphase HeLa cells, isolated gastric parietal cells, and isolated gastric glands. Immunogold labelling of H?ATPase is shown in parietal cells of isolated gastric glands embedded in LR White resin," wrote A. Sawaguchi and colleagues.
The researchers concluded: ...
- Cells can be frozen with minimal loss using high-pressure freezing, planchettes.
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...is shown in parietal cells of isolated...the fact that parietal cells cultured for...suspensions to high-pressure freezing specimen planchettes...Methods, High Pressure Freezing, Aluminum Planchettes, Parietal Cells, Gastric Glands...
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