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Aspergillus niger effective host for virus-like particle production.
2003 DEC 3 - (NewsRx.com & NewsRx.net) -- Scientists have demonstrated that Aspergillus niger is an effective host for virus-like particle production, using the hepatitis B surface antigen as a model.
"The filamentous fungus Aspergillus niger was transformed with the hepatitis B virus S gene encoding the major viral envelope protein under control of the constitutive A. nidulans glyceraldehyde-3-phosphate dehydrogenase (gpdA) promoter. Approximately seven copies of the expression cassette were integrated on the genome, resulting in high-level transcription of the S gene," researchers in South Africa report.
"Production of the 24-kDa S protein and a 48-kDa S protein dimer in the membrane-associated protein fraction of the recombinant A. niger strain was shown through Western analysis," said Annette Pluddemann and Willem H. van Zyl at the University of Stellenbosch. "Electron microscopy of partially purified recombinant S protein revealed the formation of spherical pseudoviral particles with a diameter of 22 nm. The production level of hepatitis ...
- Circadian rhythm of Aspergillus niger and Penicillum notatum: a comparative...
- Magazine article from: Journal of the Alabama Academy of Science Ssenkoloto, Margaret Adams, Sonya Williams, Tiffany Carwell, Marcus October 1, 2002 700+ words
...Hyphomycete fungi, Penicillium notatum and Aspergillus niger, to demonstrate differences in their...Penicillium notatum (29-7968 B251) and Aspergillus niger (29-7872) purchased from Carolina...impacting on the human biological clock. Aspergillus niger and Penicillium notatum are ...
- EnviroLogix Announces the First Rapid On-Site Test For Detecting Stachybotrys...
- Press release article from: Business Wire March 24, 2003 700+ words
...QuickTox(TM) Kit for Stachybotrys and Aspergillus niger, an on-site, fast, reliable...method for detecting Stachybotrys and Aspergillus niger mold spores in dust and air handling...know quickly if Stachybotrys and Aspergillus niger spores are present in inhabited buildings...
- Independent Laboratory Testing Shows BioNeutral's Hospital Grade Ygiene(TM)...
- Press release article from: PR Newswire July 7, 2009 700+ words
...performed by ATS Labs shows that Aspergillus Niger mold spores are completely...approximately 2.5 million Aspergillus Niger spores were totally eliminated within two (2) minutes. Aspergillus Niger is a type of black mold spore...
- Purification and thermodynamic characterization of glucose oxidase from a newly...
- Magazine article from: Canadian Journal of Microbiology Bhatti, H.N. Madeeha, M. Asgher, M. Batool, N. June 1, 2006 700+ words
...extract of a locally isolated strain of Aspergillus niger NFCCP. The enzyme was partially...assays. Key words: glucose oxidase, Aspergillus niger, kinetics, thermodynamics, thermal...une souche isolee localement de Aspergillus niger NFCCP. L'enzyme fut partiellement...
- PCR analysis can aid Aspergillus niger endocarditis diagnosis in AML patients.
- Newspaper article from: Health & Medicine Week January 19, 2004 700+ words
...published research from Wales, "Endocarditis secondary to Aspergillus niger has not been described in a leukemic patient." D. McCracken...chain reaction aids in the diagnosis of an unusual case of Aspergillus niger endocarditis in a patient with acute myeloid leukaemia...
- Method for purification of Aspergillus niger xylanase developed.
- Newspaper article from: Obesity, Fitness & Wellness Week June 26, 2004 700+ words
...for simultaneous renaturation and partial purification of Aspergillus niger xylanase. "Three-phase partitioning (TPP) is carried...for simultaneous renaturation and partial purification of Aspergillus niger xylanase. BBA-Proteins Proteomics, 2004;1698(1...
- Dietary Aspergillus niger phytase increases iron absorption in humans
- Magazine article from: The Journal of Nutrition Sandberg, Ann-Sofie; Hulthen, Lena Rossander; Turk, Maria February 1, 1996 700+ words
...measured from the same kind of rolls supplemented with phytase-deactivated wheat bran and with added microbial phytase from Aspergillus niger (A. niger)(4) to the rolls. Microbial phytases work in a broader pH area than cereal phytases. According to Simell...
- Xspray Expands to Meet Pharma's Interest in its Ground-Breaking Particle...
- Newspaper article from: Biotech Week May 13, 2009 700+ words
...AB, the Swedish life science technology company, has developed a cutting-edge solution for pharmaceutical particle production based on supercritical fluid technology. The Company's RightSize Particle Manufacturing Technology overcomes...
Source: HighBeam Research, Aspergillus niger effective host for virus-like particle production.