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Bacterial protein produced in starch granules of transgenic maize kernels.

Vaccine Weekly

| November 19, 2003 | COPYRIGHT 2003 NewsRX. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan.  All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)Copyright

2003 NOV 19 - (NewsRx.com & NewsRx.net) -- Bacterial proteins were produced in starch granules of transgenic maize kernels.

"The B subunit of Escherichia coli heat labile enterotoxin (LT-B) is a potent oral immunogen with potential for use as a vaccine, a carrier molecule to deliver antigens to gut-associated lymphoid tissues, and possibly an adjuvant to make co-administered vaccines more effective. LT-B produced in plants was shown to be functional and immunogenic in animals and humans. In this work, we show that maize-derived LT-B is strongly associated with starch granules in endosperm," scientists in the United States report.

"Using immunogold labeling/electron microscopy, cell fractionation, and protein analysis techniques, we observed that LT-B protein could be detected both internally and externally in starch granules," stated Rachel K. Chikwamba and collaborators at Iowa State University and Arizona State University. "This strong association confers an effective copurification of the antigen with the starch fraction of maize ...

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Source: HighBeam Research, Bacterial protein produced in starch granules of transgenic maize...

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