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Individualized human scFv vaccines produced in plants.
2003 NOV 19 - (NewsRx.com & NewsRx.net) -- Scientists have produced individualized human scFv vaccines in plants.
"We have developed a method for rapidly producing in plants the idiotype regions of the tumor-specific Ig as single-chain Fv (scFv) proteins for use in the treatment of non-Hodgkin's lymphoma. Variable region gene sequences were generated from either a tumor hybridoma or human tumor biopsy cells, and idiotype domains were joined by a novel linker and cloned into a modified tobacco mosaic virus (TNW) vector designed to secrete the scFv protein in infected Nicotiana benthamiana plants," researchers in the United States report.
"Thirty-eight out of 44 human scFv proteins showed Coomassie visible material in crude secretory (interstitial fluid, IF) extracts, 21 of those between 100 and 800 microgram/mL," said Alison A. McCormick and collaborators at Large Scale Biology Corporation and Stanford University. "Eight of these proteins were tested for appropriate idiotype responses in vaccinated mice. In all eight cases, anti-idiotype immune responses ...
- Phage-display selection technique for specific human scFv antibody...
- Newspaper article from: Cancer Weekly January 7, 2003 700+ words
...colleagues concluded: "Hence, we have demonstrated that a synthetic G(M3) panning reagent can be used to isolate a fully human scFv that is highly specific for native G(M3) on the surface of tumor cells. The result is a significant step toward effective...
- Phage-display selection technique for specific human scFv antibody described.
- Newspaper article from: Immunotherapy Weekly January 8, 2003 700+ words
...colleagues concluded: "Hence, we have demonstrated that a synthetic G(M3) panning reagent can be used to isolate a fully human scFv that is highly specific for native G(M3) on the surface of tumor cells. The result is a significant step toward effective...
- New findings from General Hospital, Department of Dermatology describe advances...
- Newspaper article from: Biotech Week June 17, 2009 700+ words
...the report 'Secretory expression of human ScFv against keratin in Pichia pastoris and...cycle analysis, Which indicated that human ScFv against keratin 17 can inhibit the proliferation...Dermatological Research (Secretory expression of human ScFv against keratin in Pichia pastoris ...
- Researchers at Nanjing Medical University publish new data on life...
- Newspaper article from: Biotech Week August 27, 2008 700+ words
...attenuated by interaction with its specific human scFv E3 in vitro,' is the subject of a report...results indicate that site-directed human scFv might be a potential therapeutic agent...attenuated by interaction with its specific human scFv E3 in vitro. Life Sciences, 2008...
- Scientists at Ewha Womans University publish new data on enzyme...
- Newspaper article from: Biotech Week May 13, 2009 700+ words
...optimization and engineering." "In this study, a fully synthetic human scFv library with six diversified CDRs was constructed by polymerase...in Molecules and Cells (Construction of a large synthetic human scFv library with six diversified CDRs and high functional diversity...
- New findings reported from Tsinghua University describe advances in Alzheimer...
- Newspaper article from: Obesity, Fitness & Wellness Week May 2, 2009 700+ words
...Alzheimer Disease). "Here, we report four single-chain variable fragment (scFv) antibodies isolated from the naive human scFv library by phage display that specifically recognized A beta oligomers but not monomers and.brils. These conformation...
- Endothelial cell-specific human antibodies might retard tumor growth.
- Newspaper article from: Biotech Week June 30, 2004 700+ words
...Germany, "we have isolated single-chain Fv fragments directed against human endothelial cells from a novel fully synthetic human scFv library (scFv 479). This library was constructed using the variable germline segments DP47 and DPkappa9 as scaffolds...
- Novopharm Biotech's Anti-Cancer Drug H11 (NovoMAb-G2scFv) Reduces the Formation...
- Press release article from: Business Wire April 4, 2000 700+ words
...product candidate H11 may bring to patients with breast cancer." The presentation entitled, "Effect Of An Unconjugated Human scFv Antibody Fragment On Growth And Metastasis Of A Breast Adenocarcinoma In Athymic Mice," is being presented as a poster at...
Source: HighBeam Research, Individualized human scFv vaccines produced in plants.