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Orthopoxviruses detected using microarrays of immobilized oligonucleotides.
2003 NOV 12 - (NewsRx.com & NewsRx.net) -- Researchers report the detection and discrimination of orthopoxviruses using microarrays of immobilized oligonucleotides.
"Variola virus (VARV), causing smallpox, is a potential biological weapon. Methods to detect VARV rapidly and to differentiate it from other viruses causing similar clinical syndromes are needed urgently. We have developed a new micro array-based method that detects simultaneously and discriminates four orthopoxvirus (OPV) species pathogenic for humans (variola, monkeypox, cowpox, and vaccinia viruses) and distinguishes them from chickenpox virus (varicella-zoster virus or VZV)," scientists in the United States and Russia report.
"The OPV gene C23L/B29R, encoding the CC-chemokine binding protein, was sequenced for 41 strains of seven species of orthopox viruses obtained from different geographical regions," said Majid Laassri and collaborators at the U.S. Food and Drug Administration and the State Research Center of Virology and Biotechnology 'Vector' in Russia.
"Those C23L/B29R sequences and the ORF 62 sequences from 13 strains of VZV (selected from GenBank) were used to design oligonucleotide probes that were immobilized on an aldehyde-coated glass surface (a total of 57 probes)," the authors ccontinued. The microchip contained several unique 13-21 bases long oligonucleotide probes specific to each virus species to ensure redundancy and robustness of the assay."
"A region approximately 1,100 bases long was amplified from ...
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Source: HighBeam Research, Orthopoxviruses detected using microarrays of immobilized...