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Genetic analysis of reassortants of strains of influenza B virus reported.
2003 NOV 5 - (NewsRx.com & NewsRx.net) -- Scientists have published the results of a genetic and phenotypic analysis of reassortants of high growth and low growth strains of influenza B virus in a recent issue of the journal Vaccine.
According to a study from the United States, "The yield of influenza virus in eggs is critical to influenza vaccine production and availability, but the contribution of specific genes to the growth properties of influenza B viruses is not well understood. Influenza B/Beijing/184/93 and B/Shangdong/7/97 were chosen for study because B/Shangdong/7/97 replicated to several-fold higher titers in eggs than B/Beijing/184/93 as demonstrated by hemagglutination titers and EID[subscript]50."
"A reassortant with the HA, NP, and PB2 genes from B/Beijing/184/93 and all other genes from B/Shangdong/7/97 had the high growth phenotype of B/Shangdong/7/97 in eggs, which suggests that NS, M, NA, PBI or PA, or a combination of these genes derived from B/Shangdong/7/97 were needed for the high growth phenotype of the reassortants," reported G. M. Vodeiko and colleagues at the United States Food and Drug Administration. "A high degree of homology was found among the genetic sequences of B/Beijing/184/93, B/Shangdong/7/97, and other influenza B ...
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